Has anyone tried cross-linking cells with formaldehyde before co-immunoprecipitation?

Hi, I am currently trying to co-immunoprecipitate a protein but the interaction between that protein and its binding partner is not stable. Sometimes we able to detect its binding partner but...

29 November 2020 9,261 3 View

How to fix half the molecules in an ensemble while annealing?

I created a rectangular ensemble consist of crystalline erythritol molecules. I want to create a liquid-solid interface through annealing, making half of the ensemble amorphous and keep the other...

27 September 2020 2,632 1 View

GROMACS How do I apply position restraints on erythritol molecules?

I was trying to apply restraints on my molecules, however, they were still moving after I put 100000kJ/mol/nm^2 on them. I am sure my topology is correct, so I think maybe its the correlation of...

23 September 2020 3,958 3 View

TOP/FOP-FLASH assay in HEK293T cells?

Has anyone tried to study Wnt signaling in HEK293T cells? I have been trying to measure the Wnt activity using TOP/FOP-FLASH assay but the readings that I got are rather similar in both TOP and...

28 August 2020 6,052 4 View

A question about western blot?

I have run western blots for 25 years. Today I got this strange blot using Aviva OAPB01225 klotho antibody and 1% BSA in TBST to block for 1 hour. It doesn't matter whether I load 10 ug or 50 ug...

31 December 2019 9,605 3 View

Loading control for membranbe proteins?

I need to study cell membrane protein by western blots but encounter a problem with adequate loading control. Any suggestions?

07 August 2019 3,010 5 View

How mitochondria mediates fatty acid metabolism in cancer cells?

We just identified a mitochondrial protein that regulates fatty acid metabolism (FAM) in cancer cells. Does somebody have expertise in FAM? For example, which kit(s) or method(s) can be used to...

01 January 2019 4,187 3 View

Calculation of drugs concentration from IC50(in vitro) to CED(in vivo) injection?

Convection-enhanced delivery (CED) system is a powerful treatment for directly bypass BBB in brain tumor therapy. I try to set up the system and the in vivo animal model. In the begin, we use TMZ...

26 June 2018 7,719 3 View

Do we have unimolecular organic reaction during 350K for kinetic study?

30 January 2018 7,970 2 View

Any need to do unit root test for very short time series?

I read that the validity of unit root test becomes very small if the number of observations is small. In this case, it is very likely to have Type II error, say wrongly accepting the null...

08 January 2018 1,505 11 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View

Could someone help me troubleshoot my NOS assay protocol for skeletal muscle extracts, please?

I am trying to measure differences in eNOS activity in skeletal muscle in sedentary and exercised mice, following the conversion of 3H-arg to 3H-cit, and assaying the samples with and with out...

31 January 2021 8,324 1 View

No signal/bands observed on western blot for ERK signaling?

My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...

30 January 2021 1,039 3 View

What is the best way to minimize/prevent the effects of rigor mortis in excised mouse muscle?

I'm planning to excise mouse muscle to use for ex vivo mechanical testing. What is the best way to prevent the stiffening effects of rigor mortis from confounding results if I cannot isolate the...

30 January 2021 1,641 2 View

Need for help. What goes wrong with my differentiatio process of C2C12?

Hello, I'm now working on C2C12 myoblast cell line. Growing the cells was great, but there are some problems during differentiation process. The cells are not differentiating but frequently...

30 January 2021 9,535 2 View

Any good methods for double-stained markers quantifications from tissue sample using computer programs?

How can we best quantify double-stained markers when comparing diseased and control? For example, if we want to stain one marker of smooth muscle cells and compare the expression in diseased and...

21 December 2020 7,389 1 View

How I Can calculate the resistance of each vessel in vascular system based on inlet pressure condition and outlet flow rate?

I have an ordinary vascular system, where I know the inlet condition of pressure on the system and value of flowrate through each outlet. Based on these two pieces of information I need to...

16 December 2020 1,599 1 View

Does increasing the number of individuals by copy-paste of the same indvs to perform GWAS affect the results?

I am trying to perform a genome-wide association on mitogenome variants (obtained from GATK-Mutect2) vs. phenotypes by using GAPIT. The number of individuals is seven and GAPIT function does not...

14 December 2020 8,853 3 View

Is there any tool to extract phenotypes from facial photos?

The facial photos corresponds to various rare genetic disorders.

22 November 2020 738 1 View