Ru Nm

5 Questions 8 Answers 0 Followers

Questions related from Ru Nm

Why i can't see the protein in the gel?

I am working on a small protein (15 KD) ; their is no information about its function ; we can't see it in western blot and we stain it but we cant see in the gel (or maybe can see but we sisnt...

24 January 2024 6,599 10 View

Two proteins in western blot

Hi I am working on lair2 and it's interaction with C1q to understand who that affect lupus. We used cell line to produce lair2 . than we transfected it with c1q. and now we are doing western blot...

01 January 1970 5,915 1 View

Two proteins in western blot

Hello I am working on c1q and lair2 and how their interaction affects lupus (autoimmune disease) . we used western blot , yet we can't see lair 2 ,

01 January 1970 766 1 View

Small wells in western blot

Hi I am working on polyacrylamide gel for western blot. I don't know why I get small wells . when I prepare the gel it sound good , yet when I load the samples it is small.

01 January 1970 5,470 4 View

I can't see my protein in the gel - western blot?

Hi to check if the protein exist in the cells and the medium ( cell culture and transfection), we conduct western blot . at the start we saw the protein yet when we repeat the experiment with...

01 January 1970 1,204 3 View