Ru Nm

7 Questions 8 Answers 0 Followers

Questions related from Ru Nm

What is the size of c1q in wb?

Hi I am using c1740 - c1q - and testing it in WB - I use anti c1q-c. Human complement C1q has a molecular mass of 460 kDa. C1q is composed of 18 polypeptide chains, 6 each of 3 different...

11 September 2024 9,071 0 View

Why i can't see the protein in the gel?

I am working on a small protein (15 KD) ; their is no information about its function ; we can't see it in western blot and we stain it but we cant see in the gel (or maybe can see but we sisnt...

24 January 2024 6,620 10 View

Two proteins in western blot

Hi I am working on lair2 and it's interaction with C1q to understand who that affect lupus. We used cell line to produce lair2 . than we transfected it with c1q. and now we are doing western blot...

01 January 1970 5,939 1 View

Two proteins in western blot

Hello I am working on c1q and lair2 and how their interaction affects lupus (autoimmune disease) . we used western blot , yet we can't see lair 2 ,

01 January 1970 788 1 View

Small wells in western blot

Hi I am working on polyacrylamide gel for western blot. I don't know why I get small wells . when I prepare the gel it sound good , yet when I load the samples it is small.

01 January 1970 5,493 4 View

I can't see my protein in the gel - western blot?

Hi to check if the protein exist in the cells and the medium ( cell culture and transfection), we conduct western blot . at the start we saw the protein yet when we repeat the experiment with...

01 January 1970 1,230 3 View

How can I remove non specific binding from my control sample

Hi I am working on Lair2 - small protein that can connect to C1q and MBL (complement system) . I have three samples , WT, MUT and GST(as control), we used GST beads and incubate them ( WT, MUT...

01 January 1970 4,843 0 View