What is the role of APS in sds page & can it be substituted by any another reagent?

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Davide Magrì Popular answer

Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide. Usually when the APS can not be used, Riboflavin is suitable as a photopolymerization reagent in PAGE, but there are some variations in the protocol.

Davide Magrì

Hardik Manek

APS is an oxidizing agent which spontaneously decomposes to form free radicals and is used with TEMED to catalyze the Polymerization of acrylamide and bisacrylamide monomers.

Michael B LoMonaco

Kudos to D. Magri for relating this old and valuable B vitamin initiator technique. It is a way to preserve sensitive epitopes and enzyme activity.

Ram Saran Chaurasiya

yes i too agree with these answers....but i don't know if there is any other chemical which can be used as substitute for this...

J.P. Mandal

APS or ammonium persulfate is the one which causes polymerization of the Acrylamide and bisacrylamide when acted upon by the TEMED (TetraMethylEthyleneDiamine) and the polymerization is based on a free radical mechanism. TEMED reacts with the APS splitting the APS into the sulfate free radical which then initiates the polymerization of the Acrylamide and Bisacrylamide.

Kenneth Omabe

Excellent explainations ..No idea if there are substitutions

Jannah N.M.S

can i ask something out of scope? is the reaction of APS and TEMED also applicable to production of hydrogel using biopolymers? Because, we can see in most literature that the combo of APS and TEMED specifically for the polymerization of acryl amide and acrylic acid, both being synthetic polymers. some research made use of APS but without TEMED on the grafting of synthetic polymers aforementioned onto pectin backbone (pectin is long chained polysachharide from the peeling of citrus and apple pomaces, used as food thickener). so is it possible for APS and TEMED to react on mere biopolymers, with no addition of any monomers of synthetic origin. thanks if anyone can help me on this

Sarwar Jyoti

If i use less APS in gel plate is there any problem for western blot

Athira Mohandas Nair

APS is a oxidising agent that provides free radicals along with Tetraethylmethrylenediamine fo polymerizing acrylamide