J.P. Mandal

5 Questions 10 Answers 0 Followers

Questions related from J.P. Mandal

What could be the reason for the observation of two opposing phenotypes (cell arrest and cell migration) in cancer cell?

I observed growth inhibition/cell arrest coupled with cell migration at the same time during treatment of drug X that activates MAPK signaling. Why are cancer cells arrested before they could...

26 February 2020 3,736 5 View

How much Coverage is required for NGS (amplicon) using Ion Torrent PGM system to detect SNPs, indels?

I want to do DNA mutation analysis by NGS using Ion Torrent PGM sequencing instrument. I will make use of "Ion Ampliseq cancer hotspot panel v2" to amplify cancer mutation hotspots. May I know...

15 May 2017 3,056 3 View

How effective is Ion AmpliSeq™ Cancer Hotspot Panel v2?

How effective and reliable would "Ion AmpliSeq™ Cancer Hotspot Panel v2" be for identifying variants of oncogenes and tumor suppressors genes in xenograft tumor? Expecting comments from users of...

24 April 2017 7,605 2 View

What is the necessity of 5' or 3' RACE?

In what situation do we go for 5' RACE or 3'RACE and what are the informations these experiments can provide?

22 December 2015 4,611 2 View

How can I make a HEX labelled primer to work?

I optimized and got desired band with unlabeled primer set. But when i use labeled primer with HEX (Left primer labeled) i'm not able to get the band. Three other labeled (other labels) primers...

02 June 2015 9,086 3 View