I want to know the required thickness of a 3D scaffold for tissue engineering.
There is no specific thickness required. It depends on what you are studying. If the scaffold is too thick, for example, oxygen and nutrient diffusion and therefore cell survival at the centre of the scaffold will be poor.
Hi,
it will strongly depend on your application (in vitro test, in vivo implantation?) cells you want to grow in/on, mechanical properties you aim to achieve and the chemical nature of your scaffold! If your strategy is to mimic the ECM of the regenerating tissue for example, you should find relevant data on the ECM composition and its specific properties/activities of the tissue you want to regenerate and try to mimic them to get a biomimetic material.
It really does depend on the type of cells you are trying to seed as well as the application and potentially whether you want to trap any cells in or out of the matrix while allowing diffusion of certain agents.
There are a lot of good resources to read on the matter, but I suggest you start here:
http://www.math.uci.edu/~lowengrb/RESEARCH/publications/TissueEng.pdf
Best of luck!
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