After cutting bands having low molecular weight peptides obtained from SDS-PAGE,is it possible to elute these peptide(s) without digesting in trypsin?

Basically, I want to get the sequence of peptides. I assume, digesting these peptides from each band may give me a different sequence than the original through LC-MS analysis. If digestion doesn't...

30 January 2021 5,482 3 View

How many kind of Contrastive loss are there? What is the significance of each kind?

Hello all, It would be kind of you to help me find out what are the different types of contrastive loss functions that currently available and their significance regarding different aspects i.e....

27 September 2020 2,993 2 View

How Hansen solubility parameters helps in drying fibers during electrospinning?

Hansen solubility parameters determine hoe a polymer is soluble in a the ifferent solvent. But how it helps in case of solidifying a fiber during electrospinning process?

14 September 2020 4,570 2 View

Is it possible to use MAC address of a device as an authentication key for accessing a file?

is it possible to use a MAC address of device as an authentication key for accessing a file? can this be implemented manually ? like if I want a specific device to access a file( i have no access...

22 July 2020 2,667 15 View

Can two index number be employed in Gmsh?

Hi I am trying to build an square lattice array of perforation on a disk surface. I like to create a for loop inside a for loop. One for x positions (loop 1) and other for a y position for all the...

17 June 2020 2,610 1 View

What kind of assessment can be conducted to find the changes in terms of protease or proteolytic activity?

I want to observe the changes in terms of protein breakdown during germination. Few techniques are known such as Colorimetric, fluorescence assay to assess protease activity. Which one is the best...

23 January 2020 9,487 1 View

How does even Majorana Fermion exist ?

How does even Majorana Fermion exist ? Why any fermion can be written as a combination of two Majorana fermions? Is there any physical meaning in it? Why Majorana fermion can be used for...

26 January 2019 3,017 3 View

What will be the net force equation for a SISO radial AMB system?

I have a SISO system of active magnetic bearing, i/p of which is current and output is the combine displacement in x and y. the normal equation for force of both axis is like this :...

19 August 2018 6,129 1 View

Decrease of Corss Polarization Level?

What is the procedure to reduce the cross polarization level for a given antenna? Is there any general guideline?

08 May 2017 9,946 1 View

'Biomedicine Spray-On RNA Protects Plants from Viruses for Weeks' a news in nature plants 9-1-17. Will not RNase enzyme be a problem there?

RNase enzymes are everywhere. Therefore, there should have possibilities to degrade RNA that are sprayed to plant.

18 January 2017 3,450 7 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

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19 February 2021 8,647 3 View