what is the recommended dosage of nisin and natamycin in fruit juices? Are there any FSSAI or FDA regulations?
Natamycin is reported to be most effective against yeast at between
20 and 50 ppm (Delves-Broughton et al., 2006). Nisin used in the most of articles between (1 : 2.5 ppm). Nisin is widely used as preservative as it can inhibit a wide range of bacteria in food. It is affirmed by US FDA as GRAS (generally recognized as safe) and widely accepted as safe food additive in many countries with E number E234 .
Nisin was affirmed GRAS by Food and Drug Administration in 1988 (FDA, 1988), and now used as a biopreservative in 57 countries around the world. Because it is non-toxic, heat stable and does not contribute to off-flavours, nisin is commercially used in a variety of foods including dairy, eggs, vegetables, meat, fish, beverages and cereal-based products to inhibit growths of foodborne pathogens including Listeria monocytogenes (Schillinger et al., 1996).
That's all i know about Natamycin and Nisin ,,,, Good luck.
For Natamycin
The Joint Expert Committee on Food Additives (JECFA) established an acceptable daily (ADI) intake of 0.3 mg/kg bw/day based on human clinical studies. The level causing no toxicological effects in man was estimated to be 200 mg/per/day, equivalent to 3 mg/kg bw/day.
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