What is the reason for change in mRNA level of specific gene but not change in protein level?

With treatment of cell lines ( cardiofibroblast cells), changes in mRNA level of the gene of interest has been observed overtly, but there is no change in protein level. I am wondering how this...

07 August 2017 1,964 7 View

How can point mutation in a protein affect amount of other protein in cells?

It has shown that about gene knock out in mice'brain, the amount of other protein can be affected (Maybe in mRNA level or protein stability). I want to know whether point mutation can lead to the...

04 May 2017 3,735 4 View

Have anybody experience in taking special GRE in biochemistry?

I'm looking for a set of books that I can read in preparation for the GRE Subject Test In Biochemistry, Cell And Molecular Biology

08 September 2015 8,014 1 View

Can anybody introduce books in field of oncology?

I want to know oncology books that is comprehensive. I want to know from beginner to advanced. I need materials that is useful for molecular medicine major.

06 July 2015 8,964 3 View

Does rigidity structure of enzyme always lead to decrease enzyme activity?

Article show that rigidity affects enzyme activity and high rigidity causes low activity. Is there any exceptions? How does increasing rigidity cause high activity?

01 February 2015 9,685 11 View

What is the effect of immidazole on activity and stability of enzyme?

When enzymes were eluted with high concentration immidazole, some of them lost their activity in 2 days. I want to know how immidazole affect enzyme or other protein. I can't find article about that.

31 December 2014 8,559 7 View

How does the removal of a small domain from an enzyme affect the optimum PH of activity?

my enzyme is cellulase. It has two domains( small domain and catalytic domain). small domain has a few hydrophobic interaction with catalytic domain. I determined PH optimum for truncated and...

11 December 2014 8,513 4 View

How can I assay the aggregation of my enzyme?

I removed small domains from my enzyme. I want to know which one (truncated or wild-type) is more likely to aggregate at different times at 65 centigrade. Can you give me a strategy to go about...

11 December 2014 5,926 9 View

Why do truncated and wild-type enzyme have different colors in bradford assay with the same sharpness in SDS-PAGE?

I remove 85 aa from my enzyme(cellulase). I purified enzymes(truncated and wild-type). After dialysis, I run 20 microliter of them for SDS-PAGE. Both of them have the same sharpness on gel, but...

10 November 2014 7,843 4 View

Why did my protein elute with wash buffer (even 10 mM imidazole)?

I can't purify my protein with the ni-agarose column. The PH wash and elution buffer is ok but all of my protein eluted in the wash buffer. What is my problem?

09 October 2014 981 17 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View