Parastou Rahimizadeh

11 Questions 37 Answers 0 Followers

Questions related from Parastou Rahimizadeh

I want to know how i can be certain about formation of inclusion body during protein expression?

I want to express a recombinant form of protein in bacteria and i'm not sure about this protein expression is soluble form. I want to know is there any way to be certain that it will be expressed...

09 September 2019 9,213 10 View

How can monitor how much studies are done on special matter?

I want to know when rate of application special kind of treatment started and how much studies exctlly are done on this matter . Actually is there any website to show rate of publication about...

09 September 2018 8,300 3 View

Does the enzyme immobilized on nano carrier based on FTIR spectra?

I want to immobilize my enzyme on ZIF-8 with covalent and entrapment techniques.I use DCC (dicyclohexyle carbodiimide)for carrier activation. Does this FTIR spectra prove immobilization?

06 June 2018 436 0 View

How can one reduce As+3 to As0 without interference of As+5?

When we have two forms of As in an environment, how and in which potential does the reduction of As+3 to As0 occur, and the interference of As+5 form is zero?

02 February 2015 3,987 3 View

Can anyone help me with FTIR interpretation?

I immobilize enzyme on chitosan macrobead with Glutaraldehyde as crosslinker. I want to know how FTIR confirm immobilization? Which wavelength is related to chitosan with glutaraldeyde and which...

01 January 2015 2,703 11 View

Can a circular dichroism monitor immobilization effect on protein structure?

When you immobilized enzyme on polymeric support can you detect changes in protein content (αhelix% or β sheet% in enzyme)?

01 January 2015 9,638 6 View

Why doesn't my protein have desirable activity?

I work on cellulase enzyme and express it in E.coli(BL21). In SDS-PAGE I have good bands, but after purification with Ni-NTA column and dialysis, it's activity is very low (according to enzymatic...

06 June 2014 6,181 16 View

What is my problem in cloning?

I want to clone my gene to expreesion vector in Ecoli, after I clean my pcr product, I have a good band. In digestion step, after digesting the gene I don't get any band, but I perform a ligation...

02 February 2014 5,319 17 View

I do gel cracking for separate bands, unfortunately I only see a smear in each well. Why only the weak gene band is visible?

I reculture each colony in another plate after transformation and use them for cracking in the cracking buffer (SDS,NaOH,EDTA,D2O), I run them with 10 micro dye hardly but I don't see plasmid...

01 January 2014 8,298 1 View

Can someone advise me why my protein fails to express?

I work on eukaryotic gene, I want to clone and express it, I transform my recombinant plasmid to Ecoli(BL21), after induction, my protein does not express. I don't see my protein band in SDS...

12 December 2013 4,777 7 View

Why is my PCR band is so weak?

I do PCR reaction for my gene (it's about1600bp). Nonspecific bands are sharper than my my specific band. I examined temperature gradient, different template concentration (template is vector...

12 December 2013 1,902 16 View