Currently I am doing SDS PAGE. After adding all solutions, polymerization did not occur. I never encountered this problem previously. I prepared every solution freshly with correct procedure. I tried with different volumes of APS and TEMED also. But still no use. What could be the reason for this? Can anyone help me to troubleshoot this problem?
Did you de-gas the acrylamide solution before adding APS? what percentage gel were you making?
hello Prem
some of the things that i have troubleshooted are
1) Need acylamide and bis-acrylamide(29:1)
2)Old APS or APS not properly stored.
If u have added bis-acrylamide try to use new APS and try it.
You may change your APS & TEMED and try now. Some times the TEMED might have been oxidized.
As suggested try fresh APS and TEMED. This is normally the reason polymerisation does not occur. Failing that try new Acrylamide bis
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