Nithin Sam .R

11 Questions 47 Answers 0 Followers

Questions related from Nithin Sam .R

Delta delta Ct analysis for prokaryotes?

What is the significant fold increase for delta delta ct analysis for a prokaryotic gene. Is it above 2 fold? If so, have any one have got above two fold increase in any prokaryotic gene? Iam...

12 December 2016 5,488 4 View

Is fold difference and fold change cut off the same in real time PCR?

For significant change it was said FC should be above 2. Is it the same for prokaryotic and Eukaryotic expression analysis? Can some one brief me on this issue and whether it have any relation...

07 July 2016 8,141 2 View

How to know whether the result obtained from qPCR are significant or not?

Is their a particular fold difference or any statistical method to find whether the q pcr result for a treated sample and untreated sample are significant or not?

07 July 2016 5,602 5 View

Will Total RNA get amplified using dna specific primer?

will rna get amplified when using it as a template for PCR? -using dna specific primer -with gc% 55 -using high fedility polymerase.

06 June 2016 4,424 8 View

Will endogenous control vary between different bacterial isolates of same organism?

Let as say their is a susceptible isolate and a resistant isolate, the resistant isolate have more acquired resistant genes. To express those genes more ribosomes are needed and so more...

06 June 2016 536 4 View

What is the best gene knockout method in clinical isolates of Pseudomonas and Acinetobacter?

I need to knockout some genes for functional studies. can any one suggest me the best and time saving technique which i can use. One problem i would face is the isolates are resistant to most of...

06 June 2016 2,586 3 View

Suggestion regarding mutation analysis in TB?

My friend want to design a study to find the mutation in TLR2, NRAMP-1, VDR and IFN gamma-R using RFLP technique. If any one have experience in it can you please suggest the primers and...

04 April 2016 4,212 0 View

Can any one suggest the integrity of the RNA gel pic that i have attached?

I want to know whether the RNA i have isolated from Klebsiella pneumoniae is in good condition. I would also like to know what the four bands are (there should be only 3 bands and 5s is not...

04 April 2016 1,237 6 View

Why annealing temperature changes for a same bacteria but different isolate?

When i did PCR for a house keeping gene for different isolate of Acinetobacter baumannii i got no band and a less band for two isolates. Then i did a gradient PCR to get intense band on both....

04 April 2016 4,021 2 View

How can I analyse codon bias in case of silent mutation in a house keeping gene in microorganisms?

I would like to understand the reason behind the silent mutation and nonsense mutation in house keeping genes. Since I am working with multidrug resistant bacteria, i would like to know if there...

03 March 2016 6,290 5 View

Regarding MLST (Multi Locus Sequence Typing) data in Acinetobacter baumannii using 7 house keeping genes?

I have done MLST for some isolates of Acinetobacter baumannii which are obtained from ICU scenario in a single month. In which five genes have same clonal number in all isolates while only two...

03 March 2016 7,040 2 View