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Questions related from Nithin Sam .R
What is the significant fold increase for delta delta ct analysis for a prokaryotic gene. Is it above 2 fold? If so, have any one have got above two fold increase in any prokaryotic gene? Iam...
12 December 2016 5,502 4 View
For significant change it was said FC should be above 2. Is it the same for prokaryotic and Eukaryotic expression analysis? Can some one brief me on this issue and whether it have any relation...
07 July 2016 8,160 2 View
Is their a particular fold difference or any statistical method to find whether the q pcr result for a treated sample and untreated sample are significant or not?
07 July 2016 5,615 5 View
will rna get amplified when using it as a template for PCR? -using dna specific primer -with gc% 55 -using high fedility polymerase.
06 June 2016 4,439 8 View
Let as say their is a susceptible isolate and a resistant isolate, the resistant isolate have more acquired resistant genes. To express those genes more ribosomes are needed and so more...
06 June 2016 552 4 View
I need to knockout some genes for functional studies. can any one suggest me the best and time saving technique which i can use. One problem i would face is the isolates are resistant to most of...
06 June 2016 2,601 3 View
My friend want to design a study to find the mutation in TLR2, NRAMP-1, VDR and IFN gamma-R using RFLP technique. If any one have experience in it can you please suggest the primers and...
04 April 2016 4,227 0 View
I want to know whether the RNA i have isolated from Klebsiella pneumoniae is in good condition. I would also like to know what the four bands are (there should be only 3 bands and 5s is not...
04 April 2016 1,252 6 View
When i did PCR for a house keeping gene for different isolate of Acinetobacter baumannii i got no band and a less band for two isolates. Then i did a gradient PCR to get intense band on both....
04 April 2016 4,037 2 View
I would like to understand the reason behind the silent mutation and nonsense mutation in house keeping genes. Since I am working with multidrug resistant bacteria, i would like to know if there...
03 March 2016 6,305 5 View
I have done MLST for some isolates of Acinetobacter baumannii which are obtained from ICU scenario in a single month. In which five genes have same clonal number in all isolates while only two...
03 March 2016 7,060 2 View