What is the reason for big white pellet problem in plasmid isolation?

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Do I require Informed consent for observational clinical investigation of Medical device which is already commercially present in investigation site?

Hi, I am planning to conduct a clinical investigation as per ISO 14155:2020 for one of our products for which the target population is neonates. Our product is already commercially available on...

07 February 2022 2,347 6 View

How many defuzzification methods are there in the literature? Which of them is the best?

I know some defuzzification methods: signed distance, graded mean integration, centroid, regular weighted point method and ranking method. What is the purpose/advantages of these methods? Whether...

05 October 2021 1,748 3 View

What are the advantages of using different fuzzy numbers (triangular, trapezoidal, pentagonal, etc.) in the inventory model?

I think so that it may depend on the nature of the uncertainty.

19 September 2021 913 4 View

Can floc characterization be done using UV spectrophotometer?

Am doing my research project in turbidity removal in synthetically prepared water using algal alginate - a natural coagulant. I need to characterize the floc formed. Is it possible to go for floc...

13 March 2019 3,570 1 View

Can we use glass sleeve as an alternative for quartz sleeve?

I am planning to design a lab scale UV reactor for sulphate radical based advanced oxidation of paper mill effluent. Can I go for glass sleeve instead of quartz sleeve for the UV lamp ? Can anyone...

24 January 2019 8,553 3 View

Effluent characteristics of Paper mill using waste paper as raw material?

Can somebody give me general range of COD and BOD values in the effluent of paper and board mills using waste paper as raw material?

22 January 2019 6,665 5 View

How to perform reclass in IDRISI in order to use the output as input in CellAtom module?

Our current work involves landuse prediction using CA-Markov model in IDRISI. As an intermediate step, suitability image is to be created using CellAtom option in IDRISI. One of the inputs in...

21 September 2016 5,787 2 View

Is there any model to evaluate suitablity of groundwater quality for irrigated agriculture?

I have done geochemical analysis for groundwater samples and obtained results of major cations and anions. In order to evaluate the suitability of groundwater quality for various crops, I am...

16 August 2016 9,784 4 View

What is a good extraction method of Autologous Thrombin from Whole Blood by Manual method?

I am looking for a working protocol for the production of autologous thrombin from whole blood by manual method? Thank you in Advance !!

20 July 2015 2,175 7 View

Is it feasible to obtain 100 Million Mesenchymal Stem Cell in a 2 Layered Cell Factory?

I would be using 2 layered cell factory (1272cm2) for Mesenchymal stem cell culture. Source - P1 Umbilical cord Mesenchymal Stem Cell. If it is feasible to culture and arrive at the magical...

29 June 2015 8,286 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View