Can we use glass sleeve as an alternative for quartz sleeve?

I am planning to design a lab scale UV reactor for sulphate radical based advanced oxidation of paper mill effluent. Can I go for glass sleeve instead of quartz sleeve for the UV lamp ? Can anyone...

24 January 2019 8,511 3 View

What all thing we should keep in mind while selecting a solvent for hydrothermal reaction in a 50 ml autoclave?

I am going to use a 50 ml teflon lined autoclave for doing hydrothermal synthesis and I am deeply concerned about its safety. The pressure limit inside the autoclave is 3MPa and temperature...

14 February 2018 7,313 3 View

What is the reason for big white pellet problem in plasmid isolation?

In plasmid DNA isolation by alkali lysis method, I get big white pellets after isopropanol washing. It is not dissolving and it also not resolving in gels afterwards. Please any suggestions.

17 December 2017 8,213 2 View

Are there any distinct morphological characters by which Anopheles subpictus species B can be distinguished from other sibling speices?

The available morphological character for distinguishing adults of species B is the lengths of apical white band and subapical dark band of female palpi. However, there are reports of variations...

25 January 2017 1,400 4 View

Is it possible to isolate/detect Japanese Encephalitis virus from Bird droppings?

Migratory birds are likely to transport JE viruses from an endemic area to a non-endemic area. Screening their blood would be the best method to detect JE virus in them. However, it is not...

01 April 2015 8,156 12 View

How can I add manually digitized drainage networks in the ArcSWAT model?

I have created drainage network automatically using ArcSWAT model and DEM with

05 May 2014 3,401 3 View

What is the current status of the Zika virus in Asia?

26 February 2014 7,707 3 View

Does anybody know various theories on the origin and evolution of insects?

In our entomology syllabus one of the components is origin and evolution of insects-various theories. Can anybody suggest a suitable book/paper dealing with this issue?

25 November 2013 4,229 3 View

Can anybody identify the species?

It was found inside an unopened packet of Killer Zone trilogy CD for PS3 brought from the US.

22 November 2013 9,553 14 View

T-test as a statistic for the analysis of fMRI data - can anyone help?

Why are t-tests preferred over other statistical tests for the analysis of fMRI data?

05 October 2013 8,594 8 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View