How can writing tasks be designed or redesigned to accommodate the diversity in a multi -level ESL classroom?

Designing writing tasks in level- playing conditions of diverse groups in ESL settings calls for structural adjustment with the established notions of task design features. Yet , the culture of...

21 October 2020 9,469 6 View

How to Convert large fastq file to fasta?

Hello, I'm new to bioinformatics, my NGS dataset consists of 50 FASTQ files and each file is of size 10-15 GB.. I need to convert these FASTQ files to FASTA files. P.S. I am using google colab I...

02 July 2020 6,224 2 View

How can I Optimize Deep Learning Model such that its cost/size is reduced with little accuracy spike?

Let say I have a trained alexNet model, I want to optimize it to inference i.e, post-training optimization. So far I found "quantization" that is also available in tensorflow. What are other...

23 January 2020 5,282 2 View

RNA Quantity & Quality extracted from Escherichia coli in M9 supplemented with different sugars?

I am trying to extract RNA from Escherichia coli grown in M9 minimal media supplemented with different sugars, however, every time the RNA extract ranges from 25 to 50 ng/ul. I am using the Qiagen...

23 October 2018 992 2 View

Is there an online source to know the equivalence of each OD to cfu/ml for Escherichia coli?

Is it the same for all media or differs from LB to minimal media? Doesn't OD correlate to cell mass? To be sure should i do a viable count for the growth of E.coli in specific media?

20 October 2018 2,588 3 View

Best Gel documentation system ?

02 April 2018 6,383 4 View

Electroporation of cripr cas9 by gene pulser?

Kindly I need to do transfection for canecr cell lines by Crispr cas 9 from santa cruiz (downregulation and overexpression) , I wonder if cuvette 0.2 will work with it as I don't have 0.4 and the...

28 March 2018 3,398 2 View

Size of electroporator cuvette size for mammalian transfection ?

I am trying to transfect SNU449 cell line by GFP plasmids using genepulserX Bio rad electroporator. I wonder if I can use .4 cm cuvette and voltage needed ! and what is the cell size of SNU449 !

21 March 2018 7,864 3 View

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I am trying to do transfection of SNU 449 hepatocellular carcinoma cells by GFP plasmids using Bio Rad micropulser electroporator ! according to its protocol it nables safe and reproducible...

13 March 2018 8,271 3 View

How to combine Response spectrum analysis results with static structural results in ansys workbench ?

I am working on load combination effects on structure and i need to combine the results of response spectrum analysis with static structural ... How can i achieve the desired results ?

10 October 2016 3,322 4 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View