I have been working on the anti-teratogenic activity of a plant extract towards ethanol induced duck embryos however, many studies use the 14 days incubation period and then remove the embryo for morphological analysis. In my case, I want the eggs to hatch and use Tona's degree of malformation scoring table. What would be the best time to inject the teratogen and the anti-teratogen? Is it Day 0 of incubation?
Article Effect of carbon dioxide during the early stage of duck egg ...
check this article
I do not have an answer for you but a suggestion. Why don't you inject on day 0, 7, 14 etc., and compare the results? Why would you only focus on one day and leave the rest? Suppose there is a 'day' effect that actually varies?
Kindly check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5603876/
Also check https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957076/
https://www.researchgate.net/deref/https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC5603876%2F
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