You can determine that by flow cytometry.You can triple stain your cells with CD90,CD34 and CD31 to determine the CD90 population and the hematopoietic lineage/endothelial lineage cells.Remember to compensate the wavelengths before triple staining.

I think that you're looking for USSC cells which these most distinctive characteristics of CD45- and positive CD105 and CD90 expression.

Thanks Charan and Saeed for you valuable inputs,

what if my cellls are are Early Endothelial Progenitors, what could be the panel of markers i should look for

are they CD 133, CD117, VEGF 2, KDR, Flk 1 and SCF

what about FGF (1 or 2)

native cells or after induction with GF?

@Saeed, You are not clear to me........

A double positive for CD34 and CD31 will give you the population of the endothelial lineage cells and CD31 alone with give a mature endothelial lineage population.So you can do a 5 color staining for flow say CD31,CD34,CD105,CD90 and CD 73.So you will get your numbers for endothelial/hematopoietic and mesenchymal cells.

@charan...thanks a lot, but i have already tried this......not coming to any conclusion

If you want to specifically detect endothelial population you can also try a very specific Von Willebrand Facotor(vWF)staining.This will surely give you the endothelial population.

@charan

thanks will surely try out this and come back to you..............

Dear Purva,

If you solely detected CD90 positive and CD34/CD45 negative cells you then isolated and culture cells derivés from some MSC-like lineage. Further pluripotency assays, e.g. towards adipogenic, chrondogenic and osteogenic lineages, will corroborate that they are unrestricted somatic stem cells or typical MSCs with more restricted pluripotency. Please, if you want, read our articles devoted to this topic in PubMed. Do not hesitate to contact me for further questions.