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Questions related from Charan Thej
I want to try using Plerixafor (AMD3100), an antagonist for CXCL12-CXCR4 binding in vitro. Could someone help me with the solvent that has to be used to dissolve and the shelf life post dilution?
21 October 2015 7,592 3 View
I am working with the effect of MSCs on the degradation of ECM components especially collagens.I am wondering if the MMPs present in the conditioned medium of MSCs can degrade collagens in an...
13 January 2014 1,335 6 View
I have homogenized cartilage from guinea pig and I would like to estimate the sGAGs for this. My question is, is it possible to estimate the GAG and normalize to total protein instead of the...
25 December 2013 402 4 View
Does anybody know where I can get a human hepatic stellate cell line?Maybe LX-1 or LX-2? Who are the vendors for these cells?
12 November 2013 9,966 7 View
Does the activation of the HSCs occur by infiltrating macrophages or is it due to an upregulation of an activator like TGF beta? On what signal do the macrophages migrate into the liver?...
04 September 2013 9,524 7 View
Is there a commercially available neutralizing monoclonal antibody for human HGF?
29 July 2013 695 1 View
Post activation of hepatic stellate cells, I would expect the accumulation of the ECM component collagen.Can the collagen be detected in the culture media?or is it in the cell lysate or extract?...
26 June 2013 6,967 0 View
What could be the mechanism by which mesenchymal stem cells would be involved in cartilage defects like osteoarthritis?Is it a percentage engraftment and forming cartilage?Or is it by a 'hit and...
25 June 2013 5,036 4 View
There could be two or three different pathways of HGF signalling: one to promote angiogenesis/survival and the other to have an antifibrotic effect (Ras-Raf-MEK-1-MAPK). In a scenario where...
27 May 2013 9,345 5 View
What could be a good in-vitro fuctional assay for chondrogenesis using stem cells?
29 April 2013 3,668 10 View