Dr.Purva Sanganeria Sethi

13 Questions 53 Answers 0 Followers

Questions related from Dr.Purva Sanganeria Sethi

Do you have knowledge on applications of magnetic nanoparticles in cardiovascular diseases?

In what all ways can magnetic nanoparticles with various biocompatible coatings be used for dealing with cardiovascular disorders? What are the newer imaging modalities which can be incorporated...

12 July 2014 4,972 1 View

Does anyone know a technique for in vitro MRI for IONPs?

What is the procedure and what type of instrumentation is required? What can the outcomes be?

02 June 2014 8,137 2 View

What are the different model systems which can be used for various in vivo analysis for an application of iron oxide nanoparticles ?

In vivo model system development for MRI scanning in mice models to test the efficacy of magnetic nanoparticles (IONPs).

27 April 2014 7,571 4 View

How do you determine the iron content in Fe3O4 using ICP-AES?

What is the minimum threshold value of Iron which should be present for further cytotoxicity analysis? Is it really trustworthy to rely on UV-Vis Spectra of iron oxide nanoparticles?

11 April 2014 4,888 10 View

How can I quantify the amount of protein coated on the surface of my magnetic iron oxide nanoparticles?

Are the techniques named as MFM (Magnetic force Microscopy), CD (Circular Dichroism), PPMS (Physical Property measurement system) sufficient to find out this particular information or are there...

29 March 2014 8,778 9 View

Future of iron oxide nanoparticles in the biomedical fields?

Biomedical applications of magnetic iron oxide nanoparticles are widely explored in the field of MRI, Hyperthermia, optics, biosensors and many more. What can be the new approach to apply these...

07 January 2014 6,653 11 View

Are pH sensitive dyes really a matter of concern for Actin cytoskeleton studies?

Some fluorescent dyes for example FITC and rhodamine are stated to be less stable in pH fluctuations. What precautions should be taken to study Actin cytoskeleton and what are the preferred dyes?

06 January 2014 3,601 0 View

Stem cell isolation from cord blood?

What are the basic protocols people are using with ficoll density gradient method, in how many days cells start appearing and taking there fibroblastic morphology, what is the ratio for the...

18 July 2013 3,201 0 View

How do you perfom a LDH cytotoxicity Assay ?

I have a Roche LDH Cytotoxicity detection kit and want to analyse the percentage of cytotoxicity, what is the role of the several background controls mentioned in the Kit protocol? what are the...

25 June 2013 3,841 1 View

What is the mechanism behind internalisation of protein coated iron oxide nanoparticle and their mode of toxicity ?

Why are the biocomaptible iron oxide nanoparticles less toxic when compared to the bare nanoparticle ? what is their mode of internalisation? Coated IONPs - internalised (phagocytosis,...

20 June 2013 936 3 View

what is the minimum therapeutic concentration of Iron (Fe) required in the form of biocompatible iron oxide nanoparticle for MRI ?

Iron Oxide nanoparticle behave as contrast agents in MRI

17 June 2013 8,391 2 View

What is the population in human umbilical cord blood samples which are expressing only CD90 and not any other hematopeitic and endothelial markers?

Cells have been isolated from human cord blood using ficoll density gradient method and are ongoing till P11

17 June 2013 7,770 10 View

What is the reason behind shrunken stem cell morphology with subsequent passaging?

What are the reasons for it and what type of precautions should be taken to prevent this type of morphology, the white granule appearance?

05 June 2013 2,275 10 View