I was wondering if there is a fixed pI for all monoclonal antibodies as most of the labs use glycine and phosphate buffer systems for MAb isolation using Protein A-Affinity (in HPLC).
No, each mAb has its particular pI. It may differ greatly between different mAbs (ranging from 6.5-7 to 8.5-9). On the other hand, Protein A-Affinity purification does not depend on the pI of the antibodies and for this reason the most common buffers systems are Phosphate buffer to equilibrate the column and Gly-ClH (at low pH) to elute the antibodies.
As Daniel mentioned the purification protocol is not based on the specific pI of the Ab. Elution is made at low pH in order to destabilize and disrupt the strong interaction established at neutral pH between protein A and Fc.