How good are the products of Originlab?

Hi all. I deal with flow cytometry data and analyze by FlowJo. In order to analyze and represent it in a better way, I would like to buy the Origin lab software (https://www.originlab.com/) which...

11 December 2018 7,422 4 View

How to visualize flow cytometry data in 3D format using FlowJo version 10.5.3?

Flow cytometry data which can be presented in various ways like dot plots, contour, density plots, etc. But in all these representations, the depth of a given population is not very clear in 2D...

10 November 2018 7,481 3 View

Cell frequency or actual cell count? Which one is more meaningful in assessing phenotype of a transgenic mouse?

Flow cytometry analysis has become a routine in now-a-days research. It is more important in hematopoiesis studies where a desired cell type, identified by a single marker or combination of...

09 October 2018 879 5 View

How to generate antibody specific to a protein which is in high similarity with another protein?

Hi everyone! I am trying to raise an antibody specific to a protein, which is highly similar and shorter in length compared to a different protein from the same family. As it makes clear I do not...

08 September 2016 2,218 6 View

How to show mean and set error bars in a vertical scatter plot or a dot plot, using Excel 2016?

I want to show the data points about the test and control samples in a vertical dot plot/scatter plot using Excel 2016. Have tried in various ways to figure it out, but could not. Can anyone...

07 August 2016 1,530 8 View

How to decrease error rate in kit-based multicolor flow cytometry analysis?

I am doing a multi-color flowcytometry analysis of mouse bone marrow cells, for which I use a kit. Each time, I need only 0.3-0.6 ul of certain antibodies. This has a chance of increasing errors...

06 July 2016 1,237 4 View

Can anyone provide a detailed vector map and size details of pARE-lux plasmid?

the details are not available in the Addgene website.

04 May 2015 2,752 1 View

What is the significance of 10^4 events in flow cytometry ? Why certain instruments like BD FACS Aria II has the lower limit of events as 10^4?

During analysis of cell suspensions expected to harbor a particular population of low number, how this 10^4 (10000) events cut-off can be managed? Starting the analysis with high number of cells...

03 April 2015 1,897 4 View

Can anyone help with my mES-OP9 co-culture experiments?

Is there any review about hematopoieitic differentiation of mouse embryonic stem cells on OP9 cells with complete profile of markers expressed? I have tried in all possible ways to find the...

03 April 2015 4,934 8 View

Can anyone suggest how to reduce precipitation in animal cell cultures?

By 2nd day of culturing HEK293 and mESCs, a lot of precipitates are found in the dish and affect the cell health badly (worse in the case of mESCs). By 4th or 5th day of culture all the cells are...

11 December 2014 4,464 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View