12 Questions 21 Answers 0 Followers
Questions related from Wulligundam Praveen
Hi all. I deal with flow cytometry data and analyze by FlowJo. In order to analyze and represent it in a better way, I would like to buy the Origin lab software (https://www.originlab.com/) which...
12 December 2018 7,456 4 View
Flow cytometry data which can be presented in various ways like dot plots, contour, density plots, etc. But in all these representations, the depth of a given population is not very clear in 2D...
11 November 2018 7,519 3 View
Flow cytometry analysis has become a routine in now-a-days research. It is more important in hematopoiesis studies where a desired cell type, identified by a single marker or combination of...
10 October 2018 913 5 View
Hi everyone! I am trying to raise an antibody specific to a protein, which is highly similar and shorter in length compared to a different protein from the same family. As it makes clear I do not...
09 September 2016 2,246 6 View
I want to show the data points about the test and control samples in a vertical dot plot/scatter plot using Excel 2016. Have tried in various ways to figure it out, but could not. Can anyone...
08 August 2016 1,566 8 View
I am doing a multi-color flowcytometry analysis of mouse bone marrow cells, for which I use a kit. Each time, I need only 0.3-0.6 ul of certain antibodies. This has a chance of increasing errors...
07 July 2016 1,267 4 View
I ve spotted a few colonies growing on Yeast extract, Peptone and dextrose (YPD) agar medium, which is exposed to a fumigated room for 3.5 hours and incubated at 30 degrees centigrade for 41...
06 June 2015 2,724 1 View
the details are not available in the Addgene website.
05 May 2015 2,822 1 View
Is there any review about hematopoieitic differentiation of mouse embryonic stem cells on OP9 cells with complete profile of markers expressed? I have tried in all possible ways to find the...
04 April 2015 4,963 8 View
During analysis of cell suspensions expected to harbor a particular population of low number, how this 10^4 (10000) events cut-off can be managed? Starting the analysis with high number of cells...
04 April 2015 1,928 4 View
By 2nd day of culturing HEK293 and mESCs, a lot of precipitates are found in the dish and affect the cell health badly (worse in the case of mESCs). By 4th or 5th day of culture all the cells are...
12 December 2014 4,497 3 View
Needed to detect a substance which is present at nanogram level on the surface of a polymer. Suggestions regarding the techniques other than the above mentioned would be appreciated.
03 March 2013 8,895 11 View
