PhD Bio renewable energy research topic?

I am having challenge in picking a research topic that focuses on bio-renewable energy probably because a lot of feedstocks and methods have been research on. I would like suggestions on topic...

27 October 2020 9,898 2 View

How to Separate saturated FFA from Unsaturated FFA?

Starting from soybean oil, how can only unsaturated free fatty acid be separated from the saturated free fatty acid in the Oil? The purpose of this is to synthesis Alkyd resin with just only the...

11 October 2020 5,149 3 View

How to syntheses Alkyd resin with bright colour from dark brown rubber seed oil?

All of the alkyd sample from rubber seed oil I have synthesized is always black in colour, such colour is not good for the production of white oil paint. I need help on what to do to achieve...

10 October 2020 9,860 1 View

Developments in South african Family law?

16 June 2020 3,683 3 View

What would be an appropriate parent-report tool for measuring circumscribed interests in young children with autism?

I'm looking to get an idea of the intensity of circumscribed interests for young children (3-6 years) with autism. Does anyone know of an appropriate parent questionnaire that can be used to...

14 January 2019 7,013 3 View

In gas-liquid-liquid STR, how is turbulence modified and what is the impact on drag felt by the gas bubbles?

In the CFD prediction of gas hold up in gas-liquid STR, it is necessary to take into account the observation that turbulence increases the effective drag felt by gas bubbles thereby reducing their...

17 June 2018 3,014 2 View

Grid size vs bubble size in Euler-Euler simulation of gas-liquid flows in stirred tank reactors?

In the simulation of stirred tank reactors, it is necessary to finely resolve the region near the impeller so as to adequately capture turbulence properties such as the energy dissipation rate....

07 February 2018 9,687 2 View

Soultion that dissolves Praziquantel powder,?

I have purchased praziquantel powder for mouse experiments. I always have a problem with dissolving this powder. I used Distilled water, hot and cold, but still no luck. Is there a proper solution...

20 May 2015 9,719 3 View

In ELISA, why does the IgG standard only bind when I coat plate with anti IgG,.,.but does not bind with my protein of interest.?

I have ran an elisa assay with one side coated with anti igg and one side coated with SEA protein,.,.i put standads in both sides,.but only the side coated with anti igg showed to have working...

16 December 2014 4,993 2 View

How can I prevent mold and yeast contamination of cell cultures?

26 November 2014 1,049 16 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

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25 February 2021 5,713 1 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View