What is the optimum time for transfection PEI system?

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Can African languages speakers export the rich cultural value their languages holds into the global linguistic market?

The intrinsic value of a language cannot be imported; however, its speakers possess the capability to export the cultural and economic value that their language holds within the global linguistic...

10 March 2024 1,776 5 View

How can African languages keep pace with modern technology and innovation terminologies without relying on transliteration?

In light of the technological advancements, the use of rigid terminologies has become an inevitable aspect. Regrettably, this has made it challenging for African languages to develop a...

25 February 2024 2,438 15 View

Why wont my Mendeley Desktop open?

Mendeley Desktop has crashed, I have downloaded and istalled it again.. Howver it cant pen. I try start mendeley desktop, the window disappears

17 May 2023 990 1 View

I want to extract bitter principles from seed coats of Strychnos spp. and determine their molecular structures.. How best can I achieve this?

Where should I start? What solvents can I use for extraction? What methods should I use to identify them? What method should I use to determine their molecular structures?

10 February 2023 2,045 2 View

What is the best antibody for Immunofluorescence of LC3 protein?

Hello, I am studying autophagy is cultured neurons and neuronal cells. I can label autophagosomes with an antibody to p62, but since it also labels aggresomes, it's not the best marker for...

06 February 2022 1,684 3 View

Safety and tolerability of antimalarials among pregnant women diagnosed with HIV?

What are some unanswered research questions on the safety / efficiency / tolerability of antimalarials, majorly artemisinin based combination therapies, use among women who are HIV positive?

18 January 2022 4,951 1 View

Can a red laser be used to perform PIV experiments?

Why are green lasers the commonly cited laser type?

09 January 2022 5,750 7 View

What can be the better way of Patients assessment to prevent hepatotoxicity of chemotherapeutics?

Many patients gets liver necrosis, and other liver toxicity suspected to have come from prescribed drugs mainly anti-drugs. There are many causes but specifically I wanted to localize on because...

26 October 2021 5,788 4 View

Research topics on carbon Carbon dioxide capture?

Please any idea of a research topic for PhD program on CO2 capture using Carbon nanotube or any other adsorbent. Or even a comparative study.

02 June 2021 1,519 4 View

How can I get source code for a published paper?

I am looking for a simulation code used for the paper Opportunistic Random access scheme design for ofdma based indoor PLC network

07 December 2020 4,571 7 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

How does social media influence people's dental habits and interest in aesthetic dental treatments?

Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...

01 August 2024 9,349 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View