Godfrey Azwindini Dzhivhuho

4 Questions 10 Answers 0 Followers

Questions related from Godfrey Azwindini Dzhivhuho

What is the optimum time for transfection PEI system?

I am transfection 293 cells for viral packaging. Will it be a problem if I change media 6 hrs post transfection. Using the PEI system.

27 October 2017 1,716 2 View

Soultion that dissolves Praziquantel powder,?

I have purchased praziquantel powder for mouse experiments. I always have a problem with dissolving this powder. I used Distilled water, hot and cold, but still no luck. Is there a proper solution...

21 May 2015 9,792 3 View

In ELISA, why does the IgG standard only bind when I coat plate with anti IgG,.,.but does not bind with my protein of interest.?

I have ran an elisa assay with one side coated with anti igg and one side coated with SEA protein,.,.i put standads in both sides,.but only the side coated with anti igg showed to have working...

17 December 2014 5,065 2 View

What cell surface markers are best in showing T and B Cell activation in mice?

I want to create a panel for T cell and B cell flow cytometry, to investigate how infection affects activation of these cells.

27 November 2014 1,580 8 View