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Questions related from Godfrey Azwindini Dzhivhuho
I am transfection 293 cells for viral packaging. Will it be a problem if I change media 6 hrs post transfection. Using the PEI system.
27 October 2017 1,813 2 View
I have Balb C mice that infected with Schistosoma mansoni, I am trying to detect IL4 cytokine using supernatants from SEA and CON a stimulated spleens using cytokine elisa, but there is nothing...
07 August 2015 7,102 3 View
I have purchased praziquantel powder for mouse experiments. I always have a problem with dissolving this powder. I used Distilled water, hot and cold, but still no luck. Is there a proper solution...
21 May 2015 9,878 3 View
If I were to inject mice with schisto eggs, I.V, will i get the systemic th2 biased response,? to an extent that spleenocytes and serum will manifest it? down-regulated ifn-g and upregulated IL4?
12 March 2015 7,149 3 View
I have ran an elisa assay with one side coated with anti igg and one side coated with SEA protein,.,.i put standads in both sides,.but only the side coated with anti igg showed to have working...
17 December 2014 5,148 2 View
A cellular protease cleaves gp160 to generate gp41 and gp120, But I also keep on seeing the gp140,.adn also gp150,. is there any structural difference between them and gp120?
12 December 2014 9,180 4 View
I want to asses substance activity in mice, but i want to know the period in which mice can be chronically infected without killing them
05 December 2014 6,496 6 View
I want to create a panel for T cell and B cell flow cytometry, to investigate how infection affects activation of these cells.
27 November 2014 1,642 8 View