Hello,
I am studying autophagy is cultured neurons and neuronal cells. I can label autophagosomes with an antibody to p62, but since it also labels aggresomes, it's not the best marker for autophagosomes.
LC3 is more specific. I have an LC3 antibody from Novus that works well for western blots. But it does not give clear labeling of autophagosomes by immunofluorescence (IF).
I'd prefer a rabbit polyclonal antibody, but a good monoclonal would also be useful. I need to order a good IF antibody as soon as possible.
Thank you,
Dr. Earl Godfrey
You can use the Rabbit Polyclonal Anti-LC3 Antibody (APG8B) (N-term) Katalog-Nummer AP1802a-ev from BioTrend. This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3. Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. Hope using this antibody you will be able to detect autophagosomes as shown in the image link given below:
See a clear labeling of autophagosomes by immunofluorescence (IF) in the link given below:
http://www.abcepta.com/assets/uploads/products/201402/AP1802a_IF_2.jpg
For more detail please see the company link:
https://www.biotrend.com/-186/rabbit-polyclonal-anti-lc3-antibody-140071156.html
Best
There are a couple of reviews at https://pabmabs.com/ which might be of interest. Just search for LC3.
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