28 September 2020 1 1K Report

I am preparing treated cells for qPCR assays. For that, I am seeding the cells in 12 well plates to add the treatment after 24 hours. I am confused about the optimum seeding density I should start with.

More Yomna Moqidem's questions See All
Why I have 2 bands at the expected molecular weight and why I have wide band?

hello everyone. Could you Please advise? I have 2 issues with my western blot:- 1) I have a wide/ broad band at the expected molecular weight (image 1) 2) I have 2 bands at the expected molecular...

22 May 2024 7,553 2 View

How to normalize a protein band to Housekeeping gene band using Biorad Image lab software?

Hello Everyone, After running the analysis of the bands using Biorad image lab software, I got values for adjusted volume and volumes for each band. Which volume (the adjusted or the second one)...

02 May 2024 2,735 3 View

What is the Estimated Protein concentration of 1 ml HMC3 cell lysate?

Hello everyone, I am currently growing Human microglia cells HMC3 in 100 mm plate. My question is what is the estimated Protein concentration (within range) of 1 ml HMC3 cell lysate? I usually...

25 April 2024 4,592 3 View

Why does my SBF ion concentration not match the iso standard?

I prepared standard SBF about 3 times and every time I perform the ICP test, ions such as Chloride are always higher than (171 mmol/L) should be and ions such as Magnesium are lower than (0.236...

04 October 2020 1,706 0 View

What dose of hydroxychloroquine sulfate should I use for in-vitro autophagy inhibition in hepG2 cells?

If I found that for chloroquine phosphate can I convert it to that for hydroxychloroquine sulfate?

28 May 2020 4,791 0 View

Could anyone help me with the detailed protocol for liver TGs assay with the lipid extraction protocol? Thanks in advance?

Using available commercial colorimetrically kit

14 June 2019 5,149 3 View

Ansys fluent CFD post error message can anyone help?

hello i am running 2D CFD simulation for wind around a square section and when i open the results (CFD-post) i get this error message " WARNING STREAMLINE:Streamline 1 Could not locate Domain...

13 February 2018 3,695 3 View

How can i be sure that all rats drink equal amount of water when i use DENA (100 mg/L) in drinking water to induce HCC in 8 weeks?

is it done by oral gavage and what is the dose/ day?

19 November 2016 7,953 5 View

Can I load equal volumes of samples that have different protein concentrations for western blot?

Hello Everyone. I have samples that significantly vary in protein concentrations. can I load equal volumes of samples that have different protein concentrations on basis that later on I will...

01 January 1970 1,926 3 View

Similar questions and discussions
Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

When should I stop watering A.thaliana for collect their seeds?

I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...

30 July 2024 2,301 7 View

How can I calculate formation energy using VASP-Ab-initio?

I would like to calculate the formation energy of P2-Na0.67Fe0.5Mn0.5O2 based on DFT, what should I do step by step. Any help would be appreciated. Thanks.

29 July 2024 8,248 2 View

How to explain the different interaction between soluble and insoluble polymer in water?

Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.

29 July 2024 6,350 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close