I am preparing treated cells for qPCR assays. For that, I am seeding the cells in 12 well plates to add the treatment after 24 hours. I am confused about the optimum seeding density I should start with.
Hi Yomna Moqidem
For RT-qPCR , seeding 150.000 SH-SY5Y cells in a 12-well plate will give a plenty of RNA.
All the best...
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