If I found that for chloroquine phosphate can I convert it to that for hydroxychloroquine sulfate?
hello everyone. Could you Please advise? I have 2 issues with my western blot:- 1) I have a wide/ broad band at the expected molecular weight (image 1) 2) I have 2 bands at the expected molecular...
22 May 2024 7,553 2 View
Hello Everyone, After running the analysis of the bands using Biorad image lab software, I got values for adjusted volume and volumes for each band. Which volume (the adjusted or the second one)...
02 May 2024 2,735 3 View
Hello everyone, I am currently growing Human microglia cells HMC3 in 100 mm plate. My question is what is the estimated Protein concentration (within range) of 1 ml HMC3 cell lysate? I usually...
25 April 2024 4,592 3 View
I prepared standard SBF about 3 times and every time I perform the ICP test, ions such as Chloride are always higher than (171 mmol/L) should be and ions such as Magnesium are lower than (0.236...
04 October 2020 1,706 0 View
I am preparing treated cells for qPCR assays. For that, I am seeding the cells in 12 well plates to add the treatment after 24 hours. I am confused about the optimum seeding density I should start...
27 September 2020 999 1 View
Using available commercial colorimetrically kit
14 June 2019 5,149 3 View
hello i am running 2D CFD simulation for wind around a square section and when i open the results (CFD-post) i get this error message " WARNING STREAMLINE:Streamline 1 Could not locate Domain...
13 February 2018 3,695 3 View
is it done by oral gavage and what is the dose/ day?
19 November 2016 7,953 5 View
Hello Everyone. I have samples that significantly vary in protein concentrations. can I load equal volumes of samples that have different protein concentrations on basis that later on I will...
01 January 1970 1,926 3 View
I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...
08 August 2024 2,298 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Reference dose and Maximum acceptable concentrations HMs
03 August 2024 8,230 4 View
I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?
02 August 2024 7,186 6 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View