What is the optimal media and culture conditions for pituitary cells in vitro culture?

29 February 2024 0 10K Report

Dear Colleagues..I need any relevant publications/ technical procedure on the optimal method for pituitary cell, gonadotropes, in vitro culture....

Badges
Science topic

More Nesrein M Hashem's questions See All

What does it mean to have negative shunt resistance when extracting the model parameters from solar I-V Curve ?

I have a negative slopes near the short circuit point on the IV curve , That maybe the reason or what ?

14 February 2024 8,491 0 View

MRI images for hepatocellular carcinoma?

I need a MRI images datasets for HCC to extract some features from it , i will be delighted if someone menstion a specific data.

16 January 2024 1,069 1 View

What are the problems of separating imprisonment and what are the solutions?

21 September 2023 299 0 View

What is the relationship between soil heavy metals and food security?

What is the relationship between heavy metals lead and cadmium in soil with food security?

29 July 2023 5,780 10 View

Could I please ask you for the latest FAO instructions in this matter (Maximum recommended dietary allowance)?

I'm writing to enquire about the latest FAO daily intake limits for heavy metals including As, Al, Cd, Cr, Cu, Hg, Pb, Fe, Ni and Zn in fish. Could I please ask you for the latest FAO instructions...

17 December 2022 4,244 2 View

I have analyzed vitamin D and its metabolites in DESI-MS and obtained a spectrum at different masses?

Now to the question, how do I know which of these masses represent vitamin D and its metabolites? Is it just a matter of freely searching for the masses of vitamin D and its metabolites and...

05 November 2022 5,636 1 View

What is the difference between DESI-MS and TOF-SIMS?

Which method is better to analyze for example Vit D and its metabolites?

23 September 2022 2,090 4 View

Has anybody done persulfate method for TN measurement in low range sample with YSI reagents?

I want to do the total nitrogen (TN) tests on water samples. My samples are all low range which means the TN concentration in them are between 0-25 mg/L. I got the prepared reagents and vials from...

07 August 2022 6,064 0 View

How to correct serial correlation in Error Correction Model?

My Error Correction Model suffers from serial correlation. How can I remove it?

31 December 2021 8,894 0 View

Following a one-way ANOVA, should I report the results for within groups statistics while I do not have any variables like male or female?

my data is gained from three groups of different nationalities but within each group I do not have any subcategories or variables for further studying the variance in the groups, mainly because...

18 August 2021 1,556 5 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,822 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,032 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 725 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 588 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,249 2 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,039 1 View