Does lipopolysaccharide from different strains of E.coli elucidate different response in different types of cells?

I would like to know if LPS from different E. Coli strains elucidates different response in different cell lines/ cell types i am working with the BV2 cell line, murine microglis cell line, and...

11 December 2015 9,785 2 View

Can I use different cells to set up compensation controls for flow cytometer?

I was wondering if I would be able to use BV2 cell line to set up the compensation controls for apoptosis assay on flow cytometer. I am staining the cells with annexin and PI. I would like to...

11 December 2015 1,328 5 View

How would I use phagocytosis assay using e.coli Bioparticles on flow cytometer?

11 December 2015 4,041 8 View

Has anybody had issues using absolute counting beads to count cells in mouse peripheral blood?

Hi, I am using Absolute counting beads to count the number of neutrophils in mouse peripheral blood. I was wondering if somebody has done similar work. I am using Count Bright beads from...

10 November 2015 6,457 7 View

What is the best way to isolate neutrophils from bone marrow?

I was wondering what is the best way to isolate neutrophils from the bone marrow without activation. what is the approximate number of cells will I get from one mouse 8-12 weeks? there is a couple...

09 October 2015 5,663 5 View

How do I stain myeloperoxidase in neutrophil?

I used neutrophil elastase antibody to stain brain sections and got a lot of background staining. I used 3% H2O2 and 2% serum blocking.

09 October 2015 6,207 3 View

What can I use to stimulate neutrophils for apoptosis?

08 September 2015 1,094 6 View

Why do I get uneven bands in Electrophoresis/ Western blot?

02 March 2015 1,800 13 View

What causes actin to "spread over" on Western blot?

Hello my dear fellows, My Beta-actin for Western started to look a little strange. The bands look wide and kind of spread over, instead of being more narrow and compact. I am not sure what causes...

11 December 2014 1,435 10 View

Why would Total Tau antibody not work on Western blot?

11 December 2014 8,133 3 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View