Does lipopolysaccharide from different strains of E.coli elucidate different response in different types of cells?

I would like to know if LPS from different E. Coli strains elucidates different response in different cell lines/ cell types i am working with the BV2 cell line, murine microglis cell line, and...

11 December 2015 9,785 2 View

Can I use different cells to set up compensation controls for flow cytometer?

I was wondering if I would be able to use BV2 cell line to set up the compensation controls for apoptosis assay on flow cytometer. I am staining the cells with annexin and PI. I would like to...

11 December 2015 1,328 5 View

How would I use phagocytosis assay using e.coli Bioparticles on flow cytometer?

11 December 2015 4,041 8 View

What is the optimal culture media for BV2 cells?

11 December 2015 8,552 3 View

Has anybody had issues using absolute counting beads to count cells in mouse peripheral blood?

Hi, I am using Absolute counting beads to count the number of neutrophils in mouse peripheral blood. I was wondering if somebody has done similar work. I am using Count Bright beads from...

10 November 2015 6,457 7 View

What is the best way to isolate neutrophils from bone marrow?

I was wondering what is the best way to isolate neutrophils from the bone marrow without activation. what is the approximate number of cells will I get from one mouse 8-12 weeks? there is a couple...

09 October 2015 5,663 5 View

What can I use to stimulate neutrophils for apoptosis?

08 September 2015 1,094 6 View

Why do I get uneven bands in Electrophoresis/ Western blot?

02 March 2015 1,800 13 View

What causes actin to "spread over" on Western blot?

Hello my dear fellows, My Beta-actin for Western started to look a little strange. The bands look wide and kind of spread over, instead of being more narrow and compact. I am not sure what causes...

11 December 2014 1,435 10 View

Why would Total Tau antibody not work on Western blot?

11 December 2014 8,133 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View