17 Questions 28 Answers 0 Followers
Questions related from Aleks Po
I would like to know if LPS from different E. Coli strains elucidates different response in different cell lines/ cell types i am working with the BV2 cell line, murine microglis cell line, and...
12 December 2015 9,855 2 View
Hi, I'm currently using DMEM with 10% HI FBS. Recently seeing no LPS response after treating the cells for 24 hours. I used the media without Hepes. Found the thread that the media needs to be...
12 December 2015 8,633 3 View
I was wondering if I would be able to use BV2 cell line to set up the compensation controls for apoptosis assay on flow cytometer. I am staining the cells with annexin and PI. I would like to...
12 December 2015 1,443 5 View
Hi, I would like to use Vybrant phagocytosis assay, using fluorescently labeled e.coli Bioparticles, to measure phagocytosis in BM derived neutrophils. I would like to read the samples on the...
12 December 2015 4,128 8 View
Hi, I am using Absolute counting beads to count the number of neutrophils in mouse peripheral blood. I was wondering if somebody has done similar work. I am using Count Bright beads from...
11 November 2015 6,533 7 View
I used neutrophil elastase antibody to stain brain sections and got a lot of background staining. I used 3% H2O2 and 2% serum blocking.
10 October 2015 6,284 3 View
I was wondering what is the best way to isolate neutrophils from the bone marrow without activation. what is the approximate number of cells will I get from one mouse 8-12 weeks? there is a couple...
10 October 2015 5,746 5 View
Hi I would like to measure apoptosis in murine neutrophils isolated from blood on flow Cytometry i am using the Annexin/PI kit from Invitrogen Initially, I would like to see what is the...
09 September 2015 1,167 6 View
Hi Fellow Colleagues: Lately I ve been having a problem with electrophoresis My bands across the gel run uneven: some are normal, others are narrow or wide. All solutions are doublechecked,...
03 March 2015 1,941 13 View
Hi Colleagues, I just did a western today to look at Phospho Tau and Total Tau protein levels in hippocampus of 3TgAD mice and met some complications [Phospho Tau Pierce MN #1020; Total Tau...
12 December 2014 8,197 3 View
Hello my dear fellows, My Beta-actin for Western started to look a little strange. The bands look wide and kind of spread over, instead of being more narrow and compact. I am not sure what causes...
12 December 2014 1,512 10 View
Yellow Western Experts! My last blot came out looking like this wonderful picture. It is absolutely maddening, I dont know whats causing this crazy background. I do the exact same thing and...
10 October 2014 5,714 9 View
Hi, I did the Ponceau on the last run, and it came out looking like these two images, attached. What could cause this, besides bad gel? Thanks.
10 October 2014 3,112 19 View
Hi, I have recently encountered a loading problem with one of my blots. The data just didnt make any sense. I reprobed for actin. The loading problem should be correctable for actin normalization...
09 September 2014 6,373 4 View
Hi, Recently one of my blots came out looking like this, I am trying to figure out whats causing this problem, the concentration of the antibody or something else. My blotting protocol is 3x...
09 September 2014 3,903 14 View
All I want is a beautiful western. Protein bands are not seen on an H-Bond PVDF membrane after transfer. Molecular markers transfer partially. I'm using NuSep Tris-Hepes 4-20% gels for...
06 June 2014 2,422 13 View
I am using cells that seem to have high autofluorescence. I've read that I can use one of the detectors free of staining with any antibodies to measure the amount of autofluorescence directly. My...
06 June 2014 7,178 9 View
