What is the optimal concentration of miRNA for microinjection into porcine oocytes?

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Why it is not common to use PD speed controller (for example for flying shears in rolling mills)?

I was simulating 3 types of speed controller types for flying shears. P, PI and PD speed controller. And my question again is, what is the reason for not using PD control even if it can...

15 May 2024 4,806 3 View

Can you recommend literature about the Skoptsy sect?

The Skoptsy were a sect within the larger Spiritual Christianity movement in the Russian Empire, best known for practising castration of men and the mastectomy of women in accordance with their...

28 January 2024 4,637 0 View

How to improve adhesion of the photoresist to CaF2?

I am trying to do EBL on CaF2 using CSAR photoresist, however the adhesion seems to be very poor and in does not get spincoated in a uniform layer. How can I improve the adhesion? Or is there any...

19 January 2023 9,897 0 View

Vector Construction design help - How would you construct Inducible lncRNA expresion linked with flourescent protein ?

Hello everyone. I am trying to construct a lentiviral construct for inducible expression of long-noncoding RNAs (lncRNA) linked to the expression of fluorescent protein ( co-expression from a...

02 January 2023 3,671 1 View

Why is it necessarry to prepare nanoparticles of elemental silver if also the silver ions exhibit antibacterial activity?

It is known that upon introduction of silver nanoaprticles to bacteria, elemental silver is transformed into ionic form which exhibits the antibacterial effect. However, silver ions can be...

06 October 2022 8,095 3 View

Gaussian16 allocated memory too high compared to actual mem?

Hi, I am running some transition state calculations in Gaussian on a moderate system (~50 atoms including 1 d-shell metal). For this, my method includes: #p opt=(calcall,ts,noeigen)...

07 February 2022 2,993 5 View

Decanol in CHARMM36?

I am looking for a decanol molecule (or similarly-sized alcohol) implemented in the CHARMM36 force field for Gromacs.

20 September 2021 7,287 2 View

How to make Parent-Daughter correction work in Genie 2000?

Hello, I've encountered a problem with Parent-Daughter correction in Genie 2000 (currently using version 3.4.1), both library and sequence are set up correctly (to my best knowledge and after...

06 August 2020 4,132 0 View

How to remove residual sperms from IVF embryos?

Do you know how to remove residual sperms from porcine IVF embryos? When I removed zygotes from IVF medium and put them to cultivation medium some of residual sperms persist till higher stages...

29 April 2020 8,282 5 View

How long will dsRNA for RNAi stay stable in -20°C?

Hi everybody, I would like to know, how long will dsRNA for RNAi experiments stay stable (frozen at -20°C). Are there some better condition to store it - not frozen at -20°C but only at 4°C?...

19 March 2020 5,342 5 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Shortage Cost Hypothesis?

What can be a hypothesis related to the Stock out Cost or Shortage Cost?

29 July 2024 9,259 2 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View

How to prevent microbubbles during immunofluorescence imaging?

Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...

01 July 2024 7,694 4 View

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

What are impact of stagflation on emerging markets performance?

The emerging markets have suffered from a state of stagflation. I hope you know your expectations according to your experiences regarding the implications for the stock markets,,,, risks and...

19 June 2024 4,410 3 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

What is the best way to synthesize cDNA of miRNA; using poly A kit or stem loop PCR?

Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...

16 June 2024 9,723 2 View

Why is iPSC in bad condition?

I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...

12 June 2024 6,087 0 View

I am working on antimicrobial effect of CBD (Cannabidiol), how should I prepare the stock which dissolve in Muller Hinton agar?

I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...

10 June 2024 9,564 6 View