Matej Murín

4 Questions 2 Answers 0 Followers

Questions related from Matej Murín

How to remove residual sperms from IVF embryos?

Do you know how to remove residual sperms from porcine IVF embryos? When I removed zygotes from IVF medium and put them to cultivation medium some of residual sperms persist till higher stages...

30 April 2020 8,242 5 View

Is it possible to transfect cells with mRNA (less than 700bp) using Lipofectamine 3000?

Is it possible to transfect cells with mRNA (less than 700bp) using Lipofectamine 3000? If yes- Is there some maximum number of bp of RNA, which could be transfected using Lipofectamine 3000? I...

09 November 2018 9,736 3 View

What is the optimal concentration of miRNA for microinjection into porcine oocytes?

Our stock is 5nmol. Or is it different for each miRNA? Thank you for answers.

31 May 2018 6,796 2 View

How can I pull a holding pipette for micromanipulation (100 μm in diameter)?

We have this type of capillaries: outer diameter 1,5 mm inner diameter 1,1 mm without filament. We have this Puller: Flaming/Brown Micropipette Puller P-97 (We can change these parameters: heat,...

04 February 2015 6,754 4 View