Do you know how to remove residual sperms from porcine IVF embryos? When I removed zygotes from IVF medium and put them to cultivation medium some of residual sperms persist till higher stages except of blastocysts. It will be problematic for transcriptomics analysis, which cannot be done like this.
Great question. I'd like to know this too. My only idea, but it's a lot of trouble, would be to use ICSI, so you don't have that problem in the first place.
Hi Matej, I used to remove the excess sperm on pig zygotes by washing them 3 times in NCSU medium additioned with 0.4% BSA before transferring them in the embryo culture media.
Philippe Godin I used to wash oocytes/zygotes after IVF twice with embryo culture medium in which the concentration of BSA is 0.3%. Do you think it will better to use concentration 0.4% of BSA or it does not matter? I can try to wash them three times instead of two times. Do you think it will be better?
Matej Murín I don't think a .1% difference in BSA concentration would transform this step into a huge success. And neither washing one additional time. If you have no other solution, try it, maybe more vigorously?
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