Is it possible to transfect cells with mRNA (less than 700bp) using Lipofectamine 3000?

Is it possible to transfect cells with mRNA (less than 700bp) using Lipofectamine 3000? If yes- Is there some maximum number of bp of RNA, which could be transfected using Lipofectamine 3000? I...

08 November 2018 9,558 3 View

What is the optimal concentration of miRNA for microinjection into porcine oocytes?

Our stock is 5nmol. Or is it different for each miRNA? Thank you for answers.

30 May 2018 6,655 2 View

Someone who is able to statisticaly analyze MALDI-TOF data?

I am looking for anyone with experience with statistical comparison of MALDI-TOF data. I have no experience with analyzing profile data and I need to compare the effect of the treatment on...

19 September 2017 5,091 3 View

How to define multi volume heat fluxes within DFLUX subroutine, Abaqus?

24 May 2017 1,673 1 View

Why reporters of deviations in pharmacotherapy are not established and well paid in many European hospitals?

According to the media many fatal medical errors have been happened in many European countries. Although a 'soul' of inappropriate treatment and prescribing is well known in the literature, in a...

31 May 2016 2,834 8 View

Why and when should we combine or switch antidepressant in patients with major depressive disorder MDD?

02 January 2016 8,597 18 View

Why many Eastern European countries did not establish clinical pharmacy service in the hospitals next to the patients? Who will deal with errors??

According to the newest evidence more than 10 % of patients are admitted to the hospital, because of inappropriate prescribing and drug-related problems. More than 20 % of patients are treated...

11 October 2015 8,973 57 View

What is your opinion about quality of antibiotic prescribing within psychiatric wards and hospitals?

Prudent prescribing of antimicrobial drugs to hospital inpatients may reduce the incidence of antimicrobial drug resistance and healthcare-associated infection. Despite strenuous efforts to...

24 March 2015 3,714 14 View

Are there any studies showing age related hypertension in regular strains of mice?

In particular I'm interested if hypertension occurs in C57BL6 mice at any time after their 18th month of age. But I am also interested in other strains. If you could point me to such studies I...

04 March 2015 401 6 View

Can anyone offer us analysis of homoarginine?

We have introduced the methodology for the estimation of reactive lysine in our laboratory. In the last step determination of homoarginine is necessary. Can anyone offer us this analysis?

01 February 2015 9,714 4 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

How does GROMACS calculate the average structure of proteins?

I want to get a average structure from a pdb file containing multiple models. I used the command below: gmx cluster -f input.pdb -s topol.tpr -cutoff 20 -method gromos -av GROMACS...

25 February 2021 6,314 1 View

How to identify or create correct list from GROMACS options during analysis of trajectory ?

During topology preparation using pdb2gmx Protein_chain_A and Protein_chain_B were identified. However, when production MD was completed and I proceeded to do analysis, I find this list : Group...

23 February 2021 5,065 8 View

Why does the sum of reaction forces not match the force component of a Free Body Cut in Abaqus?

I am using Abaqus to simulate a plate with in-plane loading. The boundary conditions allow the right edge to move up and down, the displacements on the left edge are fixed. There is no rotation...

23 February 2021 711 3 View

How to construct different filler shape in MD simulations?

Is there any way to construct different initial filler shapes like triangle, square, rectangle, pillar, and tube in any simulation software.

20 February 2021 1,750 1 View