The aim is to have general overview to compare protein profiles between treated and untreated cells, without focusing on specific protein.
Its general questions also about the quality of results between the two methods?what about the total protein ID?for example if I performed the two methods for 2 protein mixtures, in one of the...
31 December 2016 4,706 3 View
I separated proteins from porcine muscle tissue, and I noticed that three spots from CK-m type protein were resolved in around 39 kDa. My question is there any species or modified form of this...
06 July 2016 7,183 3 View
I performed analysis of my proteomics data in MaxQuant to compare different samples together; I get excel sheet from protein group I would like to know what is the difference between plain...
06 July 2016 8,529 4 View
I tried to calculate the total number of oxidation peptides but I noticed that on the list same oxidation peptides were given twice or even the third time for the same peptide.What does this...
04 May 2016 1,468 2 View
I attached the graph picture.I want to color the line of each peak with a different color that I can differentiate between them.The graph copied fro PDF file.Any free software that help?Please...
04 May 2016 8,617 4 View
I performed LC-MS/MS measurements by injecting my samples on nano liquid chromatography system (nanoACQUITYy, Waters, Manchester, UK) coupled via ESI to a quadrupole-orbitrap mass spectrometer...
04 May 2016 4,711 2 View
I performed homogenization of pancreas tissue for protein extraction by using 1X PBS(phosphate buffer saline)at physiological PH,I transferred the tissue from a tube by old forceps,I notice that...
02 March 2016 2,807 3 View
I'm a co-author in one paper and I would like to use some figures from this paper in my thesis, any tips how I can write to journal and what I should write on my letter?Usually, they accept or...
01 February 2016 2,312 0 View
During writing the thesis, I'm looking for some sites or free software that can help in rephrasing some sentences used in scientific papers.Any recommendation?and is it effective to use such sites...
01 February 2016 7,322 0 View
I have used PBS with protease inhibitors as lysis buffer to extract proteins from mamalian tissue,i choosed this buffer that not contain any detergent affect on my results on mass spectrometry.Is...
01 February 2015 5,533 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View