What is the differences between bottom-up and top-down proteomics approach?

31 December 2016 4,628 3 View

Can creatine kinase M-type protein(43 kDa) be separated in 2DE in around 39 kDa?

I separated proteins from porcine muscle tissue, and I noticed that three spots from CK-m type protein were resolved in around 39 kDa. My question is there any species or modified form of this...

06 July 2016 7,107 3 View

What is the difference between LFQ intensity and plain intensity?

06 July 2016 8,224 4 View

How I can calculate the number of oxidation peptides properly from the proteome discoverer list?

I tried to calculate the total number of oxidation peptides but I noticed that on the list same oxidation peptides were given twice or even the third time for the same peptide.What does this...

04 May 2016 1,394 2 View

I have Graph which was copied from PDF file, How can I add color to the each peak line instead of black color line.Any free software?

I attached the graph picture.I want to color the line of each peak with a different color that I can differentiate between them.The graph copied fro PDF file.Any free software that help?Please...

04 May 2016 8,534 4 View

Waters nanoAcquity UPLC system given a message: system over pressure, what does this mean? and how can i solve the problem?

I performed LC-MS/MS measurements by injecting my samples on nano liquid chromatography system (nanoACQUITYy, Waters, Manchester, UK) coupled via ESI to a quadrupole-orbitrap mass spectrometer...

04 May 2016 4,630 2 View

Is there any effect of iron (Fe) if its present in the protein solution dissolved in PBS buffer?

I performed homogenization of pancreas tissue for protein extraction by using 1X PBS(phosphate buffer saline)at physiological PH,I transferred the tissue from a tube by old forceps,I notice that...

02 March 2016 2,732 3 View

Can anybody help me in letter sample for a journal asking for permission to use figures in the paper?

01 February 2016 2,196 0 View

Can anybody provide me with papers using only PBS with protease inhibitor as buffer lysis for protein extraction from mammalian tissue?

I have used PBS with protease inhibitors as lysis buffer to extract proteins from mamalian tissue,i choosed this buffer that not contain any detergent affect on my results on mass spectrometry.Is...

01 February 2015 5,466 4 View

What are the optimum parameters for sonicate protein extraction without affecting on protein samples?

I have protein extraction from mamalian tissue,I want to do sonication to get rid of DNA and RNA then I will measure protein concentration by using nanodrop,and finally I will run on SDS-PAGE. I'm...

09 October 2014 6,567 11 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View