Eric Hill

31 Questions 20 Answers 0 Followers

Questions related from Eric Hill

What is the differences between bottom-up and top-down proteomics approach?

Its general questions also about the quality of results between the two methods?what about the total protein ID?for example if I performed the two methods for 2 protein mixtures, in one of the...

01 January 2017 4,684 3 View

What is the difference between LFQ intensity and plain intensity?

I performed analysis of my proteomics data in MaxQuant to compare different samples together; I get excel sheet from protein group I would like to know what is the difference between plain...

07 July 2016 8,488 4 View

Can creatine kinase M-type protein(43 kDa) be separated in 2DE in around 39 kDa?

I separated proteins from porcine muscle tissue, and I noticed that three spots from CK-m type protein were resolved in around 39 kDa. My question is there any species or modified form of this...

07 July 2016 7,159 3 View

I have Graph which was copied from PDF file, How can I add color to the each peak line instead of black color line.Any free software?

I attached the graph picture.I want to color the line of each peak with a different color that I can differentiate between them.The graph copied fro PDF file.Any free software that help?Please...

05 May 2016 8,588 4 View

Waters nanoAcquity UPLC system given a message: system over pressure, what does this mean? and how can i solve the problem?

I performed LC-MS/MS measurements by injecting my samples on nano liquid chromatography system (nanoACQUITYy, Waters, Manchester, UK) coupled via ESI to a quadrupole-orbitrap mass spectrometer...

05 May 2016 4,688 2 View

How I can calculate the number of oxidation peptides properly from the proteome discoverer list?

I tried to calculate the total number of oxidation peptides but I noticed that on the list same oxidation peptides were given twice or even the third time for the same peptide.What does this...

05 May 2016 1,447 2 View

Is there any effect of iron (Fe) if its present in the protein solution dissolved in PBS buffer?

I performed homogenization of pancreas tissue for protein extraction by using 1X PBS(phosphate buffer saline)at physiological PH,I transferred the tissue from a tube by old forceps,I notice that...

03 March 2016 2,783 3 View

Can anybody help me in letter sample for a journal asking for permission to use figures in the paper?

I'm a co-author in one paper and I would like to use some figures from this paper in my thesis, any tips how I can write to journal and what I should write on my letter?Usually, they accept or...

02 February 2016 2,280 0 View

What are the best sites or free softwares for rephrase sentences?

During writing the thesis, I'm looking for some sites or free software that can help in rephrasing some sentences used in scientific papers.Any recommendation?and is it effective to use such sites...

02 February 2016 7,308 0 View

Can anybody provide me with papers using only PBS with protease inhibitor as buffer lysis for protein extraction from mammalian tissue?

I have used PBS with protease inhibitors as lysis buffer to extract proteins from mamalian tissue,i choosed this buffer that not contain any detergent affect on my results on mass spectrometry.Is...

02 February 2015 5,516 4 View

What are the optimum parameters for sonicate protein extraction without affecting on protein samples?

I have protein extraction from mamalian tissue,I want to do sonication to get rid of DNA and RNA then I will measure protein concentration by using nanodrop,and finally I will run on SDS-PAGE. I'm...

10 October 2014 6,644 11 View

What is the effect of nucleic acids in the determination of protein concentration by using Nanodrop? Does it give a high false result?

I obtained a protein concentration from my sample 0.55 mg/ml, after I did filtration by using Amicon Ultra 0.5 centrifugal filter I measured the concentration again by Nanodrop and the result was...

10 October 2014 5,285 4 View

Could anybody provide me with the written SOP for Waters Micromass Q-Tof Premier Mass Spectrometer?

Recently, I started working on Q-TOF. I faced a problem in sequential steps to run samples and wonder if the run of GluFib is OK or not. Therefore, I'm looking for a written SOP for Waters...

09 September 2014 2,464 5 View

Is it possible to store mamalian tissue at -80 for two weeks before doing lyophilization?

I will perform protein extraction from mamalian tissue,i stored them overnight at -80 c before doing lyophilization. My question is, will they be affected if I store them for two weeks and then I...

07 July 2014 2,758 1 View

Can anybody help for useful tips for my PhD study?

Iam PhD student in Biochemistry,i finished almost two years from my study,i still have one and half year from my grant.I had problem that i did not do anythings in my project,only few...

06 June 2014 9,607 5 View

Can anybody help me color the 2DE spots?

I performed 2DE for 4 samples and got 4 gels. I tried to color the spots in these different gels using photoshop cc software, but I could not save the colored file later. Can anyone help me color...

05 May 2014 6,806 3 View

Can anybody explain to me what could be the reasons that I could not find any differences in spots between the four gel patterns?

I performed 2DE from cancer cell line. I had 4 gels, one with control, other are treated with anti-cancer drug with 3 different incubation time but I could not find any differences between the...

05 May 2014 7,002 10 View

Can anybody explain to me why the result from BCA protein estimation is not correct between different dilutions?

I performed protein estimation using BCA FROM thermo-scientific,i did dilution 1:10,1:100,1:1000. i noticed that the three results after i multiply by dilution factor is very far from each...

04 April 2014 5,664 2 View

Which is better: MTT or cell viability assay?

I would like to determine the concentration of an anti-cancer drug on a cancer cell line to determine the concentration that I have to use to kill only half of the cells. I used a cell viability...

04 April 2014 514 23 View

Can someone advise on adding 20 nM bortezomib to a cell,and how much volume needs to be added, if culturing cells on 25 ml medium?

I have eppi of 1 mg/ml, I do not have the molecular weigh of bortezomib, I need to culture my cells in 25 ml medium and I need to treat them with 20 nM bortezomib?

12 December 2013 2,607 2 View

For cancer cell lines, is there any passage limit number at which I must stop splitting my cells, or because it's cancerous cells it's not affected?

I've reached now passage number 50 and I want to extract the protein for proteomics study

11 November 2013 5,684 4 View

Could anybody provide me with an easy protocol to check cell viability and MTT?

I would like to check cell cancer viability before and after treatment with specific drugs.

11 November 2013 554 9 View

What is the most suitable concentration and incubation time for bortezomib (PS-341) on myeoloma cell line?

The aim is to have general overview to compare protein profiles between treated and untreated cells, without focusing on specific protein.

11 November 2013 4,684 0 View

What is the suitable concentration of phosphatse and phosphorylase inhibitors which I have to use in my lysis buffer?

I need to prepare 8 M urea as lysis buffer to extract the proteins from cancer cell lines. Therefore I 'd like to know what suitable amount of cocktail inhibitors shall I use?

11 November 2013 7,385 1 View

What is the IC50 of TNF alpha on myeoloma cell lines (i.e U266) and what is a suitable incubation time?

I'm looking for the effect of TNF alpha on myeoloma cell lines by studying profiles of proteins, therefore, in this task I will take only one concentration and one incubation point.

11 November 2013 5,794 3 View

What concentration of protease inhibitors should I use with PBS as non-denaturating buffer to wash my cells?

I use protease inhibitors cocktail tablets from Roche. I need to keep my cell intact without denaturing. According to manufacturer I should use 1 tablet for each 10 ml of lysis buffer. I...

11 November 2013 7,928 3 View

What is the best concentration and incubation time for Bortezomib on myeolama cell lines to study its effect on as much protein as possible?

And any papers to support this?

10 October 2013 7,608 1 View

What is the ideal concentration and incubation time for TNF alpha to study its effect on cancer cells especially myeloma?

I intend study the effect of TNF alpha on cancer cells especially myeloma by using proteomics approaches such as Mass spec, 2DE etc.

10 October 2013 5,713 6 View

Is it important to use decontamination mycoplasma kit after thawing cells as preventable way or do I also have to check them after thawing?

I need to thaw my freezing cancel cell line,and i do not want to check the contamination of mycoplasma,therefore i want to use a clean up mycoplasma kit.

09 September 2013 324 10 View

I have lysed my cells by 8 M urea, but I could not take supernatent well due to high viscous of pellet - can anyone help?

I have lysed my cells using 8 M urea, but I noticed that the pellet is very viscous and it was hard to get rid of pellet and take supernatent. Why did this happened? and is it affected on measure...

09 September 2013 2,561 5 View

I want to use 8 M urea only as a lysis buffer for my human cell line, to avoid using any detergent, but is it effective?

I want to measure my peptide samples on mass spectrometry, therefore I want to avoid any detergent in the lysis buffer.

09 September 2013 1,111 10 View