What is the differences between bottom-up and top-down proteomics approach?

31 December 2016 4,628 3 View

Can creatine kinase M-type protein(43 kDa) be separated in 2DE in around 39 kDa?

I separated proteins from porcine muscle tissue, and I noticed that three spots from CK-m type protein were resolved in around 39 kDa. My question is there any species or modified form of this...

06 July 2016 7,107 3 View

What is the difference between LFQ intensity and plain intensity?

06 July 2016 8,224 4 View

How I can calculate the number of oxidation peptides properly from the proteome discoverer list?

I tried to calculate the total number of oxidation peptides but I noticed that on the list same oxidation peptides were given twice or even the third time for the same peptide.What does this...

04 May 2016 1,394 2 View

Waters nanoAcquity UPLC system given a message: system over pressure, what does this mean? and how can i solve the problem?

I performed LC-MS/MS measurements by injecting my samples on nano liquid chromatography system (nanoACQUITYy, Waters, Manchester, UK) coupled via ESI to a quadrupole-orbitrap mass spectrometer...

04 May 2016 4,630 2 View

Is there any effect of iron (Fe) if its present in the protein solution dissolved in PBS buffer?

I performed homogenization of pancreas tissue for protein extraction by using 1X PBS(phosphate buffer saline)at physiological PH,I transferred the tissue from a tube by old forceps,I notice that...

02 March 2016 2,732 3 View

Can anybody help me in letter sample for a journal asking for permission to use figures in the paper?

01 February 2016 2,196 0 View

What are the best sites or free softwares for rephrase sentences?

During writing the thesis, I'm looking for some sites or free software that can help in rephrasing some sentences used in scientific papers.Any recommendation?and is it effective to use such sites...

01 February 2016 7,258 0 View

Can anybody provide me with papers using only PBS with protease inhibitor as buffer lysis for protein extraction from mammalian tissue?

I have used PBS with protease inhibitors as lysis buffer to extract proteins from mamalian tissue,i choosed this buffer that not contain any detergent affect on my results on mass spectrometry.Is...

01 February 2015 5,466 4 View

What are the optimum parameters for sonicate protein extraction without affecting on protein samples?

I have protein extraction from mamalian tissue,I want to do sonication to get rid of DNA and RNA then I will measure protein concentration by using nanodrop,and finally I will run on SDS-PAGE. I'm...

09 October 2014 6,567 11 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View