What is the minimum amount of starting material required for reduced representation bisulphite sequencing?

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Can anyone tell me that where can I find the atom symmetry codes for a crystal structure?

I have crystal structure of a newly synthesized metal-organic framework. I want to know the atom symmetry codes of the asymmetric unit of the crystal. Please help me. Thank you in advance.

28 September 2021 8,031 3 View

Can anyone provide any reference for using random k-fold for more than 50 occurrence points and jackknife for less than 50 occ points?

I have been running the ENMeval package in R for the best possible MaxEnt settings. I came across a tutorial that says we should use the random k-fold data partitioning technique if we have more...

27 September 2021 1,884 1 View

Can anyone suggest some cheap GPS data loggers with atleast 2-3 days battery backup for boat surveys?

We want to conduct a boat survey and need to track the trajectory and need to register the location and time of animal sightings. The logger should have a decent battery life, at least for 3 or...

14 October 2020 5,114 8 View

Is there any MD Simulation software for a basic PC setup with specifications of 8gb RAM, 1TB ROM, Intel i7 8th gen processor?

I have a basic student PC setup with 8GB RAM,1TB HDD, Intel i7 8th gen processor. I want to do a MD Simulation of a protein- ligand complex interaction of near about 500kda with 100-120 ns, I...

22 June 2020 5,856 3 View

Where can I find the Gene info file to analyse my Affymetrix microarray data through dChip?

Dear fellow Researchers, I am currently trying to analyze Affymetrix microarray data through dChip software and I have the input files - probe sequence and CDF for Rat 230 2, yet facing issues in...

08 March 2020 7,816 3 View

What is the best anti-coagulant for blood samples so as to ensure little or no change in antigen conformation for flow cytometry?

The choice of anti-coagulant seems to be critical as I remember reading that EDTA ruins antigen conformation and is therefore not suitable for intracellular staining of cytokines.

05 February 2020 5,010 5 View

Does anyone have the program, or know how to access the data, for the AquaRod Stream Gauge?

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02 January 2019 7,046 0 View

Can anyone suggest most established photocatalyst which can be used to develop a photocatalytic membrane in industrial wastewater treatment?

I want to purify toxic industrial waste water in Durgapur city, West Bengal, India. I need a good photocabtalyst for chemical oxidation process that can degrade most of organic pollutant present...

22 December 2018 3,127 2 View

Which Titaniam precursor and method i need to select? and which dopant is going to be suitable for this purpose?

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08 October 2018 2,924 4 View

How Titaniam precursor-Dopant Precursor pair is generally chosen for doped TiO2 synthesis?

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23 July 2018 9,057 7 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

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10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

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10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How are iso-frequency contours plotted?

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07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

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07 August 2024 5,307 1 View

Liying Yan

It is not recommended using this small amount of DNA. There will be data but the reliability and reproducible of the data may not be ideal.

Snehaa V Seal

Liying Yan Thank you for your valuable@ input.

Stephen Reynolds

Hi Snehaa,

Probably late to answer this but depending on your conversion method just remember that the recovery is going to be in the ball park of 75%+ so 2ng's could be 1.5ng's after the conversion. Depending on the post conversion method for detection 2ng's may not be enough especially in a qMSP where the level of methylation may be low. and like Liying said reproduciblity may be an issue

Thanks Stephen Reynolds

No problem Snehaa V Seal , best of luck