Can I perform an RRBS for starting material as little as 2 ng? If not, what is the best method for such a small amount of starting material for DNA methylation analysis?
Probably late to answer this but depending on your conversion method just remember that the recovery is going to be in the ball park of 75%+ so 2ng's could be 1.5ng's after the conversion. Depending on the post conversion method for detection 2ng's may not be enough especially in a qMSP where the level of methylation may be low. and like Liying said reproduciblity may be an issue