The choice of anti-coagulant seems to be critical as I remember reading that EDTA ruins antigen conformation and is therefore not suitable for intracellular staining of cytokines.
Snehaa V Seal You haven't mentioned what type of antigen you want to study from blood. Therefore, you may find the following link useful for self study about the subject matter:
https://apps.who.int/iris/bitstream/handle/10665/65957/WHO_DIL_LAB_99.1_REV.2.pdf
Best wishes.
When preparing my thesis, I used Heparin in the blood collected from rabbits for the production of antibodies. In principle, it should work for antigens.
Otherwise, heparin is not recommended in the enumeration of cells because it causes their agglutination.
There is also EDTA which is used as an anticoagulant, especially in blood tubes, since it captures Ca2 + ions which are an important factor in coagulation.
The third anticoagulant is Citrate, but in my opinion it will dilute your samples.
Sunagar Raju Iqra Zaheer Nezha EL Bari thanks for your valuable inputs.
For the choice of an anticoagulant, normally you should make two tests one sample with EDTA and another with heparin so that it is well founded.
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