Hi all,
I've been working with HL-60 cells for ~2 years in our lab. I've been able to successfully differentiate them to a neutrophil-like phenotype using DMSO (1.3% final concentration, 5 days of treatment).
However, I'm having trouble finding how that method to differentiate them was discovered.
How was it discovered that DMSO differentiates these cells to a neutrophil-like phenotype?
Is the mechanism of DMSO differentiation understood?
Thanks!
Hello Drake
Please refer to the research article below. It might be of help to you to understand the mechanism of DMSO in the differentiation of HL-60 cells.
https://www.jimmunol.org/content/jimmunol/164/1/113.full.pdf
Best Wishes.
Malcolm Nobre, thanks for this answer. I had seen this paper in my search. It does help, but it doesn't answer the question fully. DMSO treatment, in this paper, induces IRS-2. Others have shown that DMSO treatment causes decreases in c-myc, which stops the proliferation as well as aids in the differentiation process.
However, the question remains how DMSO causes these changes. I'm not sure if it is well understood.
Hello Drake
Yes, you are right. I will get back to you if I find an appropriate answer.
Regards.
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