I need 25 pmol of primers for each sample, and all of my primers are currently at a concentration of 10uM. Could someone help me figure out how to do this? I know it's basic chemistry, I just need to get it right.
Thank you!
In fact: 1 pmol/uL = 1 uM, if your primers working concentration is 25uM and you have your primers stock concentration at 10uM you should multiply your primers volume by 2.5 to reach the final required concentration. Eg.: instead of putting 1 uL you will put 2.5uL of your [10uM] primers and don't forget to subtract this volume (3uL for the primers pair) from that of water.
Agreed with Aissa Saidi
Your current primer concentrations is 10µM that means primers have 10µM conc. in 1µl of your current primer solution.
We know, 1µM = 1pmol/µl
So, 1µl current primer = 10µM = 10pmol
or, 2.5µl current primer = 25µM = 25pmol
Best wishes.
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