After deproving the nitrose cellulose membrane is it give a reliable result ?

Many lab deprove the old nitrocellulose membrane and again incubated with new antibody. I am little confused about the new and reliable result by using the old nitrocellulose membrane .is it give...

04 May 2018 4,810 4 View

anybody has the experience to use the safety cabinet UV light for inducing the cellular ROS ?

I want to use the safety cabinet UV light for inducing the cellular ROS. Needs experts suggestion to use the safety cabinets UV light. is it good choice for UV induced cellular ROS ?

04 May 2018 2,213 0 View

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I already checked in Raw 264.7 macrophages cell line .But i didn't find the Nf-kb p65 phosphorylation band .if anyone has suggestion how can i get the Nf-kb p65 phosphorylation band ? 1.Should i...

02 March 2018 4,830 8 View

Problems of SDS-PAGE making gel?

Is there is any running problem within a irregular separating gel? I am having this problem during making separating gel.Sometimes it smooth and regular base but sometimes it has irregular shape....

01 February 2018 3,964 8 View

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I already checked in Raw 264.7 macrophages cell line .But i didn't find the Nf-kb p65 phosphorylation band .if anyone has suggestion how can i get the Nf-kb p65 phosphorylation band ? 1.Should i...

01 February 2018 2,212 0 View

Can anyone tell me how to cell will be prepared for antioxidant assay like Gpx ,catalase and SOD assay?

According to Company protocol i extract the cell by using the assay buffer but the antioxidant protein was too low. How can i prepare my cell extraction for assaying the antioxidant enzyme.

09 October 2017 7,493 2 View

Which fibroblast cell line should be used in the study of rheumatoid arthritis?

04 May 2017 9,896 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

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02 March 2021 3,060 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View