I want to use the normal synovial cell line in the study of Rheumatoid Arthritis. I need some convenient synovial fibroblast cell distributor.
Many lab deprove the old nitrocellulose membrane and again incubated with new antibody. I am little confused about the new and reliable result by using the old nitrocellulose membrane .is it give...
04 May 2018 4,810 4 View
I want to use the safety cabinet UV light for inducing the cellular ROS. Needs experts suggestion to use the safety cabinets UV light. is it good choice for UV induced cellular ROS ?
04 May 2018 2,213 0 View
I already checked in Raw 264.7 macrophages cell line .But i didn't find the Nf-kb p65 phosphorylation band .if anyone has suggestion how can i get the Nf-kb p65 phosphorylation band ? 1.Should i...
02 March 2018 4,830 8 View
Is there is any running problem within a irregular separating gel? I am having this problem during making separating gel.Sometimes it smooth and regular base but sometimes it has irregular shape....
01 February 2018 3,964 8 View
01 February 2018 2,212 0 View
According to Company protocol i extract the cell by using the assay buffer but the antioxidant protein was too low. How can i prepare my cell extraction for assaying the antioxidant enzyme.
09 October 2017 7,493 2 View
I want to use the 5x DMEM media for culturing the Raw 264.7 cell line with combination of hydrogen water.How can i control the media pH when i added hydrogen water.
03 April 2017 9,851 2 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
02 March 2021 3,060 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...
02 March 2021 8,437 2 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View