I want to use the normal synovial cell line in the study of Rheumatoid Arthritis. I need some convenient synovial fibroblast cell distributor.
Many lab deprove the old nitrocellulose membrane and again incubated with new antibody. I am little confused about the new and reliable result by using the old nitrocellulose membrane .is it give...
04 May 2018 4,883 4 View
I want to use the safety cabinet UV light for inducing the cellular ROS. Needs experts suggestion to use the safety cabinets UV light. is it good choice for UV induced cellular ROS ?
04 May 2018 2,282 0 View
I already checked in Raw 264.7 macrophages cell line .But i didn't find the Nf-kb p65 phosphorylation band .if anyone has suggestion how can i get the Nf-kb p65 phosphorylation band ? 1.Should i...
02 March 2018 4,891 8 View
Is there is any running problem within a irregular separating gel? I am having this problem during making separating gel.Sometimes it smooth and regular base but sometimes it has irregular shape....
01 February 2018 4,040 8 View
01 February 2018 2,281 0 View
According to Company protocol i extract the cell by using the assay buffer but the antioxidant protein was too low. How can i prepare my cell extraction for assaying the antioxidant enzyme.
09 October 2017 7,559 2 View
I want to use the 5x DMEM media for culturing the Raw 264.7 cell line with combination of hydrogen water.How can i control the media pH when i added hydrogen water.
03 April 2017 9,930 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View