After deproving the nitrose cellulose membrane is it give a reliable result ?

Many lab deprove the old nitrocellulose membrane and again incubated with new antibody. I am little confused about the new and reliable result by using the old nitrocellulose membrane .is it give...

04 May 2018 4,810 4 View

anybody has the experience to use the safety cabinet UV light for inducing the cellular ROS ?

I want to use the safety cabinet UV light for inducing the cellular ROS. Needs experts suggestion to use the safety cabinets UV light. is it good choice for UV induced cellular ROS ?

04 May 2018 2,213 0 View

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I already checked in Raw 264.7 macrophages cell line .But i didn't find the Nf-kb p65 phosphorylation band .if anyone has suggestion how can i get the Nf-kb p65 phosphorylation band ? 1.Should i...

02 March 2018 4,830 8 View

Problems of SDS-PAGE making gel?

Is there is any running problem within a irregular separating gel? I am having this problem during making separating gel.Sometimes it smooth and regular base but sometimes it has irregular shape....

01 February 2018 3,964 8 View

Can anyone tell me how to cell will be prepared for antioxidant assay like Gpx ,catalase and SOD assay?

According to Company protocol i extract the cell by using the assay buffer but the antioxidant protein was too low. How can i prepare my cell extraction for assaying the antioxidant enzyme.

09 October 2017 7,493 2 View

Which fibroblast cell line should be used in the study of rheumatoid arthritis?

04 May 2017 9,896 0 View

What is the main purpose of using of 5X DMEM media during cell culture?

I want to use the 5x DMEM media for culturing the Raw 264.7 cell line with combination of hydrogen water.How can i control the media pH when i added hydrogen water.

03 April 2017 9,851 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View