Rahima Begum

8 Questions 13 Answers 0 Followers

Questions related from Rahima Begum

After deproving the nitrose cellulose membrane is it give a reliable result ?

Many lab deprove the old nitrocellulose membrane and again incubated with new antibody. I am little confused about the new and reliable result by using the old nitrocellulose membrane .is it give...

05 May 2018 4,862 4 View

anybody has the experience to use the safety cabinet UV light for inducing the cellular ROS ?

I want to use the safety cabinet UV light for inducing the cellular ROS. Needs experts suggestion to use the safety cabinets UV light. is it good choice for UV induced cellular ROS ?

05 May 2018 2,265 0 View

Has anyone checked Nf-kb P65 phosphorylation after 1hour at 5 µg/ml concentration of LPS induction?

I already checked in Raw 264.7 macrophages cell line .But i didn't find the Nf-kb p65 phosphorylation band .if anyone has suggestion how can i get the Nf-kb p65 phosphorylation band ? 1.Should i...

03 March 2018 4,877 8 View

Problems of SDS-PAGE making gel?

Is there is any running problem within a irregular separating gel? I am having this problem during making separating gel.Sometimes it smooth and regular base but sometimes it has irregular shape....

02 February 2018 4,023 8 View

Has anyone checked Nf-kb P65 phosphorylation after 1hour at 5 µg/ml concentration of LPS induction?

I already checked in Raw 264.7 macrophages cell line .But i didn't find the Nf-kb p65 phosphorylation band .if anyone has suggestion how can i get the Nf-kb p65 phosphorylation band ? 1.Should i...

02 February 2018 2,266 0 View

Can anyone tell me how to cell will be prepared for antioxidant assay like Gpx ,catalase and SOD assay?

According to Company protocol i extract the cell by using the assay buffer but the antioxidant protein was too low. How can i prepare my cell extraction for assaying the antioxidant enzyme.

10 October 2017 7,544 2 View

Which fibroblast cell line should be used in the study of rheumatoid arthritis?

I want to use the normal synovial cell line in the study of Rheumatoid Arthritis. I need some convenient synovial fibroblast cell distributor.

05 May 2017 9,944 0 View

What is the main purpose of using of 5X DMEM media during cell culture?

I want to use the 5x DMEM media for culturing the Raw 264.7 cell line with combination of hydrogen water.How can i control the media pH when i added hydrogen water.

04 April 2017 9,904 2 View