I am using Guanidium Thiocyanate (chaotropic agent) for RNase inhibition at the time of RNA isolation. Guanidium Thiocyanate is a very strong reagent compare to Guanidium Hydrochloride. RNA is weaker than DNA. When we use this strong reagent for the RNA isolation whether it causes to denature of the RNA or not? Then why we are mostly using Guanidium Thiocyanate for RNA isolation.
Dear Niranjan Chellathurai Vasantha according to Wikipedia, "Guanidinium thiocyanate is used as a general protein denaturant, being a chaotropic agent, although it is most commonly used as a nucleic acid protector in the extraction of DNA and RNA from cells." You can find useful articles on guanidinium thiocyanate right here on RG, e.g.:
Article Evaluation of Guanidium Thiocyanate as a Chemical Mutagen on...
Article Single-step method of RNA isolation by acid guanidium thiocy...
Both articles are available as full texts.
Dear Professor Frank T. Edelmann,
Thank you so much for your kind reply. These two articles are very useful for my study.
The difference between two is functional group by which its biochemical properties will be change . In Guanidinium thiocyanate two reactive site is present that why it have capability as a chelating agent means binding will be strong from any chemical molecule ,on the other and guanidinium hydrochloride , in this hydrochloride is firmly attached for enhancing its solubility properties.
Dear Naimish Kumar Verma "In Guanidinium thiocyanate two reactive site is present that why it have capability as a chelating agent": If you mean the thiocyanate part of the compound, you are right in that the thiocyanate anion, –S–CN, has two reactive sites. However, it cannot act as a chelating agent because of its linear structure!
Thank you so much Naimish Kumar Verma for your answer.
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