Niranjan Chellathurai Vasantha

7 Questions 18 Answers 0 Followers

Questions related from Niranjan Chellathurai Vasantha

Troubleshooting Real-Time PCR: Why Does Fluorescence Decline Instead of Showing a Sigmoidal Curve?

I’m running a Real-Time PCR to amplify RNA using primers and a Cy5-labeled probe, but instead of the expected sigmoidal amplification curve, the fluorescence curve declines. What could be causing...

25 September 2024 2,973 4 View

How to remove the salt and get purified DNA in Silica Magnetic bead-based Nucleic acid extraction?

I am doing nucleic acid extraction (manual silica magnetic bead-based) from the serum sample. After the extraction, I have taken the OD 260/280 in UV spec. There is no protein contamination. But...

05 November 2020 1,471 2 View

Could you tell me the composition of the wash buffer in RNA extraction (Silica Magnetic Bead-based) and its proportions?

I am doing silica magnetic beads-based RNA isolation. I am facing some impurity issues because of washing. So kindly share some best washing buffer composition of wash buffer for especially for...

02 November 2020 6,770 2 View

What is the main difference between Guanidium Thiocyanate and Guanidium Hydrochloride?

I am using Guanidium Thiocyanate (chaotropic agent) for RNase inhibition at the time of RNA isolation. Guanidium Thiocyanate is a very strong reagent compare to Guanidium Hydrochloride. RNA is...

19 October 2020 2,445 6 View

Which is the best concentration of EDC and NHS for Antibody and Probe conjugation?

I am using the Europium Chelate probe for antibody conjugation. When I used EDC and NHS (0.14mg/ml and 1.32mg/ml) as a crosslinker, I faced a problem. I used to get a low yield of a conjugated...

25 May 2020 1,024 0 View

Can I use Phosphate Buffer for Antibody and Fluorescence conjugation?

I am going to use Phosphate Buffer (pH 6.5) is an alternative of MES buffer (pH 6.5) for Antibody and Fluorescent Microspheres conjugation procedure. The literature I am referring to mentions MES...

23 December 2019 1,044 3 View

Do I need to do a polymerization reaction under a nitrogen atmosphere?

I am polymerizing styrene with Potassium Peroxodi Sulfate and Acrylic acid by an emulsion polymerization method. The literature I am referring to mentions stirring the reagents with a nitrogen...

19 December 2019 8,374 4 View