I recently discovered that non-pepsinized collagen forms fibrils even at acidic pH and pepsinized collagen taked longer to fibrilize. Why is it so?
How to sterilize a polymer grafted with a photoinitiator before cell culture?
23 January 2024 9,548 2 View
I was performed TCID50 to test influenza A PR8 for several times. But the MDCK cell cannot survive in the negative control after the medium was changed from DMEM medium to TPCK-infection medium. I...
20 October 2023 8,847 0 View
Why my BrdU staining shows so many false positive? My protocol was: The hippocampal slices were rinsed with 0.05 M Trisbuffer (TB) incubated for 5 min at room temperature with 0.3% H2O2, denatured...
16 August 2021 8,832 3 View
i have tried modified hummer's method for the synthesis of graphene oxide by adding Graphite powder and NaNO3 in H2SO4 . In this mix KMnO4 was added and diluted with water . this mix was heated...
13 January 2021 9,622 4 View
I have already prepared a catalyst and have very good ORR performance comparing with Pt/C testing at the RRDE testing in acid solution. But I face a problem that I can't get the good result in...
11 January 2021 1,230 0 View
I deposit metal(Ag or Au ) film (100nm) on silicon and then use annealing to diffusion into silicon. An oxide layer was found between metal and silicon in TEM. Before depositing metal, I have...
15 March 2020 7,349 2 View
Reusing C18 ziptip while desalting the peptide sample before LC-MS/MS ? Hello everyone I have a question while using ziptip Do you suggest that reuse the zip after one sample binding and...
11 December 2019 8,155 3 View
hello our lab is doing anaerobic work recently in our experience color of resazurin stock is navy blue and in the anaerobic condition is colorless and there is a transition step with pink...
11 December 2019 9,424 1 View
I have a question about the electron donor and acceptor of methanogens for Hydrogenotrophic methanogens i think the donor is hydrogen and acceptor is carbon dioxide
02 December 2019 1,957 4 View
My lab is going to develop the protein technology recently Our strategy is quick simple extract to total protein from the sample and than do trypsin digestion and do LC-MS/MS to determine the...
24 November 2019 5,562 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View