hello
our lab is doing anaerobic work recently
in our experience
color of resazurin stock is navy blue
and in the anaerobic condition is colorless
and there is a transition step with pink color
in our lab, we use purging N2/CO2 while boiling water combine Na2S method
but we have weird problem
sometimes we make the substrate with light pink color, but after several days it turn to colorless
and sometimes it is colorless but after several days it turn to light pink
so i'm curious what is the redox condition when the color is pink
is anaerobic or not, is there any ORP range can recognize the pink and colorless state ?
Actually we have different kind of pink, is it mean something ?
(our ph value is around 7)
Chao-Jui
Colorless indicates the media is anaerobic and pink indicates that is was oxidized, and the media is no longer anaerobic.
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